Histological interaction of cultured endothelial cells and endovascular embolic materials coated with extracellular matrix

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  • 1 Department of Neurosurgery, Brain Research Institute, Niigata University, Niigata, Japan
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✓ This study was undertaken to evaluate the histological reaction of cultured endothelial cells to endovascular embolic materials in vitro. Endothelial cells were isolated and cultured from a canine carotid artery. Embolic materials (platinum microcoils, polyvinyl alcohol particles, silicon balloons, or silk threads), either in their normal state or after having been coated with type 1 collagen, fibronectin, or laminin, were placed on endothelial cells and cocultured for 6, 12, and 24 hours and 2, 3, 7, 14, and 21 days. The cocultures were investigated histologically using a scanning electron microscope. Endothelial cells were not found on any uncoated embolic materials, even at 21 days. On the materials coated with fibronectin or laminin, endothelial cells began to proliferate in 7 days, covering the materials extensively in 14 days. On the other hand, endothelial cells began to proliferate on the collagen-coated materials in 3 days, covering them extensively in 7 days and reaching confluence with a cobblestone pattern in 21 days. The densities of endothelial cells on collagen-coated materials were much higher than those observed on the materials coated with other extracellular matrices. Future advantages of the clinical use of collagen-coated embolic materials in interventional treatment are discussed.

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Contributor Notes

Address reprint requests to: Shinichi Tamatani, M.D., Department of Neurosurgery, Brain Research Institute, Niigata University, 1–757, Asahimachi, Niigata 951, Japan.
  • 1.

    Aymard A, , Gobin YP, & Hodes JE: Endovascular occlusion for vertebral arteries in the treatment of unclippable vertebrobasilar aneurysms. J Neurosurg 74:393398, 1991 Aymard A, Gobin YP, Hodes JE: Endovascular occlusion for vertebral arteries in the treatment of unclippable vertebrobasilar aneurysms. J Neurosurg 74:393–398, 1991

    • Search Google Scholar
    • Export Citation
  • 2.

    Brothers MF, , Kaufmann JCE, & Fox AJ, et al: n-Butyl 2-cyanoacrylate-substitute for IBCA in interventional neuroradiology; histopathologic and polymerization time studies. AJNR 10:777786, 1989 Brothers MF, Kaufmann JCE, Fox AJ, et al: n-Butyl 2-cyanoacrylate-substitute for IBCA in interventional neuroradiology; histopathologic and polymerization time studies. AJNR 10:777–786, 1989

    • Search Google Scholar
    • Export Citation
  • 3.

    Chvapil M, , Kronenthal RL, & van Winkle W Jr: Medical and surgical application of collagen. Int Rev Connect Tissue Res 6:161, 1973 Chvapil M, Kronenthal RL, van Winkle W Jr: Medical and surgical application of collagen. Int Rev Connect Tissue Res 6:1–61, 1973

    • Search Google Scholar
    • Export Citation
  • 4.

    Dawson RC, , Krisht AF, & Barrow DL, et al: Treatment of experimental aneurysms using collagen-coated microcoils. Neurosurgery 36:133140, 1995 Dawson RC, Krisht AF, Barrow DL, et al: Treatment of experimental aneurysms using collagen-coated microcoils. Neurosurgery 36:133–140, 1995

    • Search Google Scholar
    • Export Citation
  • 5.

    De Lustro F, , Condell RA, & Nguyem MA, et al: A comparative study of the biologic and immunologic response to medical devices derived from dermal collagen. J Biomed Mater Res 20:109120, 1986 De Lustro F, Condell RA, Nguyem MA, et al: A comparative study of the biologic and immunologic response to medical devices derived from dermal collagen. J Biomed Mater Res 20:109–120, 1986

    • Search Google Scholar
    • Export Citation
  • 6.

    Debrun GM, , Viñuela F, & Fox AJ, et al: Indication for treatment and classification of 132 carotid-cavernous fistulas. Neurosurgery 22:285289, 1988 Debrun GM, Viñuela F, Fox AJ, et al: Indication for treatment and classification of 132 carotid-cavernous fistulas. Neurosurgery 22:285–289, 1988

    • Search Google Scholar
    • Export Citation
  • 7.

    Dion JE, , Duckwiler GR, & Lylyk P, et al: Progressive Suppleness Persil Cather: a new tool for superselective angiography and embolization. AJNR 10:10681070, 1989 Dion JE, Duckwiler GR, Lylyk P, et al: Progressive Suppleness Persil Cather: a new tool for superselective angiography and embolization. AJNR 10:1068–1070, 1989

    • Search Google Scholar
    • Export Citation
  • 8.

    Fox AJ, & Drake CG: Endovascular therapy of intracranial aneurysms. AJNR 11:641642, 1990 Fox AJ, Drake CG: Endovascular therapy of intracranial aneurysms. AJNR 11:641–642, 1990

    • Search Google Scholar
    • Export Citation
  • 9.

    Fox AJ, , Viñuela F, & Pelz DM, et al: Use of detachable balloons for proximal artery occlusion in the treatment of unclippable cerebral aneurysms. J Neurosurg 66:4046, 1987 Fox AJ, Viñuela F, Pelz DM, et al: Use of detachable balloons for proximal artery occlusion in the treatment of unclippable cerebral aneurysms. J Neurosurg 66:40–46, 1987

    • Search Google Scholar
    • Export Citation
  • 10.

    Guglielmi G, , Viñuela F, & Dion J, et al: Electrothrombosis of saccular aneurysms via endovascular approach. Part 2: Preliminary clinical experience. J Neurosurg 75:814, 1991 Guglielmi G, Viñuela F, Dion J, et al: Electrothrombosis of saccular aneurysms via endovascular approach. Part 2: Preliminary clinical experience. J Neurosurg 75:8–14, 1991

    • Search Google Scholar
    • Export Citation
  • 11.

    Guglielmi G, , Viñuela F, & Sepetka I, et al: Electrothrombosis of saccular aneurysms via endovascular approach. Part 1: Electrochemical basis, technique, and experimental results. J Neurosurg 75:17, 1991 Guglielmi G, Viñuela F, Sepetka I, et al: Electrothrombosis of saccular aneurysms via endovascular approach. Part 1: Electrochemical basis, technique, and experimental results. J Neurosurg 75:1–7, 1991

    • Search Google Scholar
    • Export Citation
  • 12.

    Halbach VV, , Higashida RT, & Hieshima GB, et al: Transvenous embolization of dural fistulas involving the cavernous sinus. AJNR 10:377383, 1989 Halbach VV, Higashida RT, Hieshima GB, et al: Transvenous embolization of dural fistulas involving the cavernous sinus. AJNR 10:377–383, 1989

    • Search Google Scholar
    • Export Citation
  • 13.

    Higashida RT, , Halbach VV, & Dormandy B, et al: Endovascular treatment of intracranial aneurysms using a new silicon microballoon device: technical considerations and indications for therapy. Radiology 174:687691, 1990 Higashida RT, Halbach VV, Dormandy B, et al: Endovascular treatment of intracranial aneurysms using a new silicon microballoon device: technical considerations and indications for therapy. Radiology 174:687–691, 1990

    • Search Google Scholar
    • Export Citation
  • 14.

    Higashida RT, , Hieshima GB, & Tsai FY, et al: Transluminal angioplasty of the vertebral and basilar artery. AJNR 8:745749, 1987 Higashida RT, Hieshima GB, Tsai FY, et al: Transluminal angioplasty of the vertebral and basilar artery. AJNR 8:745–749, 1987

    • Search Google Scholar
    • Export Citation
  • 15.

    Hoyer LW, , de los Santos RP, & Hoyer JR: Antihemophilic factor antigen. Localization in endothelial cells by immunofluorescent microscopy. J Clin Invest 52:27372744, 1973 Hoyer LW, de los Santos RP, Hoyer JR: Antihemophilic factor antigen. Localization in endothelial cells by immunofluorescent microscopy. J Clin Invest 52:2737–2744, 1973

    • Search Google Scholar
    • Export Citation
  • 16.

    Hynes RO: Integrins: versatility, modulation, and signaling in cell adhesion. Cell 69:1125, 1992 Hynes RO: Integrins: versatility, modulation, and signaling in cell adhesion. Cell 69:11–25, 1992

    • Search Google Scholar
    • Export Citation
  • 17.

    Jaffe EA, , Hoyer LW, & Nachman RL: Synthesis of antihemophilic factor antigen by cultured human endothelial cells. J Clin Invest 52:27572764, 1973 Jaffe EA, Hoyer LW, Nachman RL: Synthesis of antihemophilic factor antigen by cultured human endothelial cells. J Clin Invest 52:2757–2764, 1973

    • Search Google Scholar
    • Export Citation
  • 18.

    Kikuchi Y, , Strother CM, & Boyer M: New catheter for endovascular interventional procedures. Radiology 165:870871, 1987 Kikuchi Y, Strother CM, Boyer M: New catheter for endovascular interventional procedures. Radiology 165:870–871, 1987

    • Search Google Scholar
    • Export Citation
  • 19.

    Kühn K: The classical collagens type I, II and III, in Mayne R, & Burgeson RE (eds): Structure and Function of Collagen Types. Orlando, FL: Academic Press, 1987, pp 142 Kühn K: The classical collagens type I, II and III, in Mayne R, Burgeson RE (eds): Structure and Function of Collagen Types. Orlando, FL: Academic Press, 1987, pp 1–42

    • Search Google Scholar
    • Export Citation
  • 20.

    Lasjaunias P, , Rodesch G, & Pruvost P, et al: Treatment of vein of Galen aneurysmal malformation. J Neurosurg 70:746750, 1989 Lasjaunias P, Rodesch G, Pruvost P, et al: Treatment of vein of Galen aneurysmal malformation. J Neurosurg 70:746–750, 1989

    • Search Google Scholar
    • Export Citation
  • 21.

    McGuire PG, & Orkin RW: Isolation of rat aortic endothelial cells by primary explant techniques and their phenotypic modulation by defined substrata. Lab Invest 57:94105, 1987 McGuire PG, Orkin RW: Isolation of rat aortic endothelial cells by primary explant techniques and their phenotypic modulation by defined substrata. Lab Invest 57:94–105, 1987

    • Search Google Scholar
    • Export Citation
  • 22.

    Ruoslahti E, & Pierschbacher MD: New perspectives in cell adhesion: RGD and integrins. Science 238:491497, 1987 Ruoslahti E, Pierschbacher MD: New perspectives in cell adhesion: RGD and integrins. Science 238:491–497, 1987

    • Search Google Scholar
    • Export Citation
  • 23.

    Taki W, , Yonekawa Y, & Iwata H, et al: A new liquid material for embolization of arteriovenous malformations. AJNR 11:163168, 1990 Taki W, Yonekawa Y, Iwata H, et al: A new liquid material for embolization of arteriovenous malformations. AJNR 11:163–168, 1990

    • Search Google Scholar
    • Export Citation
  • 24.

    Voyta JC, , Via DP, & Butterfield CE, et al: Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein. J Cell Biol 99:20342040, 1984 Voyta JC, Via DP, Butterfield CE, et al: Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein. J Cell Biol 99:2034–2040, 1984

    • Search Google Scholar
    • Export Citation

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