Expression of monocyte chemoattractant protein-1 in meningioma

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✓ Monocyte chemoattractant protein-1 (MCP-1), purified from glioma cell line (U-105MG) culture fluid, attracts monocytes but not neutrophils. Macrophage accumulation is one of the pathological features of meningioma. To investigate the mechanism of macrophage infiltration into meningioma, the expression and localization of MCP-1 in 16 cases of meningioma were studied using Northern blot analysis and immunohistochemistry. Seven of 16 meningiomas expressed MCP-1 messenger ribonucleic acid and protein, and some degree of macrophage infiltration was seen in all 16 meningiomas.

There was a relationship between MCP-1 expression and the degree of macrophage infiltration; MCP-1 was strongly expressed in meningiomas with a high degree of macrophage infiltration. Sometimes the meningioma was accompanied by perifocal edema; a correlation between macrophage infiltration into brain tumors and perifocal edema has already been reported. It was found that the degree of MCP-1 expression is not correlated with the extent of perifocal edema.

The authors' findings suggest that MCP-1 plays an important role in macrophage infiltration into meningioma.

Article Information

Address reprint requests to: Kyoichi Sato, M.D., Department of Neurosurgery, Kumamoto University Medical School, 1–1–1 Honjo Kumamoto 860, Japan.

© AANS, except where prohibited by US copyright law.

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Figures

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    Northern blot analysis of monocyte chemoattractant protein-1 (MCP-1) and 28S ribosomal ribonucleic acid (rRNA) in meningiomas. Seven cases with positive expression of MCP-1 are shown. The U-105MG cells were used as a positive control. Arrows show the position of rRNA in 28S and 18S. The degree of MCP-1 messenger RNA expression is summarized in Table 1.

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    Case 2. Left: Photomicrograph of meningioma stained by the immunoperoxidase technique with anti—human monocyte chemoattractant protein-1 (MCP-1) polyclonal antibody. The MCP-1 is seen in the cytoplasm of the meningioma cells. Right: Photomicrograph of meningioma stained by the immunoperoxidase technique with PM-2K monoclonal antibody. The rate of macrophage infiltration is 37%. Original magnification × 200.

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    Graph illustrating the relationship between the expression of monocyte chemoattractant protein-1 (MCP-1) messenger ribonucleic acid (mRNA) and macrophage infiltration. The groups positive for MCP-1 mRNA expression evidence significantly more macrophage infiltration than the group with no expression (*p < 0.05, **p < 0.01). MCP-1 mRNA expression is classified into three groups: − = undetectable; + = lower than that found in U-105MG cells; ++ = identical to that found in U-105MG cells.

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    Schematic drawing illustrating the evaluation of peritumoral edema. a × b = edema; c × d = tumor.

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    Graph showing the relationship between monocyte chemoattractant protein-1 (MCP-1) messenger ribonucleic acid (mRNA) expression and perifocal edema. Vertical bars represent the standard deviation. No correlation of MCP-1 mRNA with the edema/tumor (E/T) ratio is noted. MCP-1 mRNA expression is classified into three groups; − = undetectable; + = lower than that found in U-105MG cells; ++ = identical to that found in U-105MG cells.

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