Expression and localization of messenger RNA and protein for monocyte chemoattractant protein-1 in human malignant glioma

Restricted access

✓ Expression of monocyte chemoattractant protein-1 (MCP-1) in human glioma cell lines and surgical specimens was studied by Northern blot analysis, reverse-transcription polymerase chain reaction, in situ hybridization, and immunohistochemistry. The samples tested consisted of 11 human glioma cell lines and eight specimens of human malignant glioma (seven from glioblastomas and one from a malignant ependymoma).

Messenger ribonucleic acid (mRNA) of MCP-1 was detected by either Northern blot or reverse-transcription polymerase chain reaction analysis in all cell lines and tumor specimens examined. In vivo expression of MCP-1 mRNA and protein was found predominantly in glioma cells with large and pleomorphic nuclei rather than in areas of small nucleated glioma cells. Adjacent brain tissue did not produce a significant level of MCP-1 mRNA or protein. Tumor vessels with endothelial proliferation expressed a moderate level of MCP-1 protein. Macrophages were found among the glioma cells, and the degree of macrophage infiltration was grossly correlated with the level of MCP-1 expression. The study results suggest that MCP-1 produced by the glioma cells may mediate macrophage infiltration into the glioma tissue.

Article Information

Contributor Notes

Address reprint requests to: Hideo Takeshima, M.D., Department of Neuro-Oncology, Box 316, The University of Texas, M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030.
Headings
References
  • 1.

    Chang HCHsu FFreeman GJet al: Cloning and expression of a γ-interferon-inducible gene in monocytes: a new member of a cytokine gene family. Int Immunol 1:3883971989Chang HC Hsu F Freeman GJ et al: Cloning and expression of a γ-interferon-inducible gene in monocytes: a new member of a cytokine gene family. Int Immunol 1:388–397 1989

    • Search Google Scholar
    • Export Citation
  • 2.

    Chirgwin JMPrzybyla AEMacDonald RJet al: Isolation of biologically active ribonucleic acid from sources enriched in ribonucleases. Biochemistry 18:529452991979Chirgwin JM Przybyla AE MacDonald RJ et al: Isolation of biologically active ribonucleic acid from sources enriched in ribonucleases. Biochemistry 18:5294–5299 1979

    • Search Google Scholar
    • Export Citation
  • 3.

    Cochran BHReffel ACStiles CD: Molecular cloning of gene sequences regulated by platelet-derived growth factor. Cell 33:9399471983Cochran BH Reffel AC Stiles CD: Molecular cloning of gene sequences regulated by platelet-derived growth factor. Cell 33:939–947 1983

    • Search Google Scholar
    • Export Citation
  • 4.

    Isobe SNakane PKBrown WR: Studies on translocation of immunoglobulins across intestinal epithelium. 1. Improvements in the peroxidase-labeled antibody method for application to study of human intestinal mucosa. Acta Histochem Cytochem 10:1671711977Isobe S Nakane PK Brown WR: Studies on translocation of immunoglobulins across intestinal epithelium. 1. Improvements in the peroxidase-labeled antibody method for application to study of human intestinal mucosa. Acta Histochem Cytochem 10:167–171 1977

    • Search Google Scholar
    • Export Citation
  • 5.

    Kelly PMABliss EMorton JAet al: Monoclonal antibody EBM/11: high cellular specificity for human macrophages. J Clin Pathol 41:5105151988Kelly PMA Bliss E Morton JA et al: Monoclonal antibody EBM/11: high cellular specificity for human macrophages. J Clin Pathol 41:510–515 1988

    • Search Google Scholar
    • Export Citation
  • 6.

    Kuratsu JLeonard EJYoshimura T: Production and characterization of human glioma cell-derived monocyte chemotactic factor. J Natl Cancer Inst 81:3473511989Kuratsu J Leonard EJ Yoshimura T: Production and characterization of human glioma cell-derived monocyte chemotactic factor. J Natl Cancer Inst 81:347–351 1989

    • Search Google Scholar
    • Export Citation
  • 7.

    Leonard EJYoshimura T: Human monocyte chemoattractant protein-1 (MCP-1). Immunol Today 11:971011990Leonard EJ Yoshimura T: Human monocyte chemoattractant protein-1 (MCP-1). Immunol Today 11:97–101 1990

    • Search Google Scholar
    • Export Citation
  • 8.

    Mihara Y: Establishment and characterization of 5 cell lines derived from human brain tumors. Kumamoto Med J 39:15411986Mihara Y: Establishment and characterization of 5 cell lines derived from human brain tumors. Kumamoto Med J 39:15–41 1986

    • Search Google Scholar
    • Export Citation
  • 9.

    Miyake E: Establishment of a human oligodendroglial cell line. Acta Neuropathol 46:51551979Miyake E: Establishment of a human oligodendroglial cell line. Acta Neuropathol 46:51–55 1979

    • Search Google Scholar
    • Export Citation
  • 10.

    Nakagawa YHirakawa KUeda Ket al: [Establishment of a human ependymoma cell line.] No To Shinkei 35:2832881983 (Jpn)Nakagawa Y Hirakawa K Ueda K et al: [Establishment of a human ependymoma cell line.] No To Shinkei 35:283–288 1983 (Jpn)

    • Search Google Scholar
    • Export Citation
  • 11.

    Oppenheim JJZachariae COCMukaida Net al: Properties of the novel proinflammatory supergene “intercrine” cytokine family. Annu Rev Immunol 9:6176481991Oppenheim JJ Zachariae COC Mukaida N et al: Properties of the novel proinflammatory supergene “intercrine” cytokine family. Annu Rev Immunol 9:617–648 1991

    • Search Google Scholar
    • Export Citation
  • 12.

    Phillips JPEremin OAnderson JR: Lymphoreticular cells in human brain tumours and in normal brain. Br J Cancer 45:61691982Phillips JP Eremin O Anderson JR: Lymphoreticular cells in human brain tumours and in normal brain. Br J Cancer 45:61–69 1982

    • Search Google Scholar
    • Export Citation
  • 13.

    Pontén JMacIntyre EH: Long term culture of normal and neoplastic human glia. Acta Pathol Microbiol Immunol Scand 74:4654861968Pontén J MacIntyre EH: Long term culture of normal and neoplastic human glia. Acta Pathol Microbiol Immunol Scand 74:465–486 1968

    • Search Google Scholar
    • Export Citation
  • 14.

    Rollins BJ: JE/MCP-1: an early-response gene encodes a monocyte-specific cytokine. Cancer Cells 3:5175241991Rollins BJ: JE/MCP-1: an early-response gene encodes a monocyte-specific cytokine. Cancer Cells 3:517–524 1991

    • Search Google Scholar
    • Export Citation
  • 15.

    Rollins BJStier PErnst Tet al: The human homolog of the JE gene encodes a monocyte secretory protein. Mol Cell Biol 9:468746951989Rollins BJ Stier P Ernst T et al: The human homolog of the JE gene encodes a monocyte secretory protein. Mol Cell Biol 9:4687–4695 1989

    • Search Google Scholar
    • Export Citation
  • 16.

    Strieter RMChensue SWStandiford TJet al: Disparate gene expression of chemotactic cytokines by human mononuclear phagocytes. Biochem Biophys Res Commun 166:8868911990Strieter RM Chensue SW Standiford TJ et al: Disparate gene expression of chemotactic cytokines by human mononuclear phagocytes. Biochem Biophys Res Commun 166:886–891 1990

    • Search Google Scholar
    • Export Citation
  • 17.

    Takeshita ITakaki TKuramitsu Met al: [Characteristics of an established human glioma cell line, KNS-42.] Neurol Med Chir 27:5815871987 (Jpn)Takeshita I Takaki T Kuramitsu M et al: [Characteristics of an established human glioma cell line KNS-42.] Neurol Med Chir 27:581–587 1987 (Jpn)

    • Search Google Scholar
    • Export Citation
  • 18.

    Takeya MTsuchiya TShimokawa Yet al: A new monoclonal antibody, PM-2K, specifically recognizes tissue macrophages but not blood monocytes. J Pathol 163:3153211991Takeya M Tsuchiya T Shimokawa Y et al: A new monoclonal antibody PM-2K specifically recognizes tissue macrophages but not blood monocytes. J Pathol 163:315–321 1991

    • Search Google Scholar
    • Export Citation
  • 19.

    Tiemeier DCTilghman SMLeder P: Purification and cloning of a mouse ribosomal gene fragment in coliphage lambda. Gene 2:1731911977Tiemeier DC Tilghman SM Leder P: Purification and cloning of a mouse ribosomal gene fragment in coliphage lambda. Gene 2:173–191 1977

    • Search Google Scholar
    • Export Citation
  • 20.

    von Hanwehr RIHofman FMTaylor CRet al: Mononuclear lymphoid populations infiltrating the microenvironment of primary CNS tumors. Characterization of cell subsets with monoclonal antibodies. J Neurosurg 60:113811471984von Hanwehr RI Hofman FM Taylor CR et al: Mononuclear lymphoid populations infiltrating the microenvironment of primary CNS tumors. Characterization of cell subsets with monoclonal antibodies. J Neurosurg 60:1138–1147 1984

    • Search Google Scholar
    • Export Citation
  • 21.

    Walter SBottazzi BGovoni Det al: Macrophage infiltration and growth of sarcoma clones expressing different amounts of monocyte chemotactic protein/JE. Int J Cancer 49:4314351991Walter S Bottazzi B Govoni D et al: Macrophage infiltration and growth of sarcoma clones expressing different amounts of monocyte chemotactic protein/JE. Int J Cancer 49:431–435 1991

    • Search Google Scholar
    • Export Citation
  • 22.

    Wood GWMorantz RA: Immunohistologic evaluation of the lymphoreticular infiltrate of human central nervous system tumors. J Natl Cancer Inst 62:4854911979Wood GW Morantz RA: Immunohistologic evaluation of the lymphoreticular infiltrate of human central nervous system tumors. J Natl Cancer Inst 62:485–491 1979

    • Search Google Scholar
    • Export Citation
  • 23.

    Yoshimura TLeonard EJ: Human monocyte chemoattractant protein-1: structure and function in Baggiolini MSorg C (eds): Cytokine. Basel: Karger1992 Vol 4 pp 131152Yoshimura T Leonard EJ: Human monocyte chemoattractant protein-1: structure and function in Baggiolini M Sorg C (eds): Cytokine. Basel: Karger 1992 Vol 4 pp 131–152

    • Search Google Scholar
    • Export Citation
  • 24.

    Yoshimura TRobinson EATanaka Set al: Purification and amino acid analysis of two human glioma-derived monocyte chemoattractants. J Exp Med 169:144914591989Yoshimura T Robinson EA Tanaka S et al: Purification and amino acid analysis of two human glioma-derived monocyte chemoattractants. J Exp Med 169:1449–1459 1989

    • Search Google Scholar
    • Export Citation
  • 25.

    Yoshimura TTakeya MTakahashi Ket al: Production and characterization of mouse monoclonal antibodies against human monocyte chemoattractant protein-1. J Immunol 147:222922331991Yoshimura T Takeya M Takahashi K et al: Production and characterization of mouse monoclonal antibodies against human monocyte chemoattractant protein-1. J Immunol 147:2229–2233 1991

    • Search Google Scholar
    • Export Citation
  • 26.

    Yoshimura TYuhki NMoore SKet al: Human monocyte chemoattractant protein-1 (MCP-1). Full-length cDNA cloning, expression in mitogen-stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE. FEBS Lett 244:4874931989Yoshimura T Yuhki N Moore SK et al: Human monocyte chemoattractant protein-1 (MCP-1). Full-length cDNA cloning expression in mitogen-stimulated blood mononuclear leukocytes and sequence similarity to mouse competence gene JE. FEBS Lett 244:487–493 1989

    • Search Google Scholar
    • Export Citation
  • 27.

    Zülch KJ: Histological Typing of Tumours of the Central Nervous System. Geneva: World Health Organization1979Zülch KJ: Histological Typing of Tumours of the Central Nervous System. Geneva: World Health Organization 1979

    • Search Google Scholar
    • Export Citation
TrendMD
Metrics

Metrics

All Time Past Year Past 30 Days
Abstract Views 312 312 48
Full Text Views 128 92 1
PDF Downloads 93 54 0
EPUB Downloads 0 0 0
PubMed
Google Scholar