Mechanism of cerebral arterial contraction induced by blood constituents

Noboru Toda M.D., Ph.D. 1 , Koichiro Shimizu M.D. 1 , and Tomio Ohta M.D., Ph.D. 1
View More View Less
  • 1 Department of Pharmacology, Shiga University of Medical Sciences, Seta, Ohtsu, and Department of Neurosurgery, Osaka Medical College, Takatsuki, Japan
Restricted access

Purchase Now

USD  $45.00

JNS + Pediatrics - 1 year subscription bundle (Individuals Only)

USD  $505.00

JNS + Pediatrics + Spine - 1 year subscription bundle (Individuals Only)

USD  $600.00
Print or Print + Online

✓ In helically cut strips of cerebral, coronary, mesenteric, and renal arteries from dogs, a test solution containing hemoglobin (Hb) and soluble constituents, obtained by exposure of blood clot to distilled water, produced a dose-related contraction. The contraction of cerebral arteries was greater than that of the other arteries. Methemoglobin (metHb) caused only a slight contraction. The contractile response of cerebral arteries to the Hb-containing test solution was attenuated by aspirin (10−5 to 2 × 10−4 M), polyphloretin phosphate (PPP, 3 × 10−5 gm/ml), a prostaglandin (PG) antagonist, and cinanserin (10−7 M), a serotonin antagonist. Combined treatment with these antagonists suppressed the response to the test solution, but only slightly attenuated contractions induced by K+. Phentolamine was ineffective. Contractions of mesenteric arterial strips induced by the test solution were potentiated by aspirin, but were attenuated by PPP and cinanserin. Serum also produced contractions of cerebral and mesenteric arteries, which were attenuated by treatment with cinanserin or PPP. Contractile responses to Hb-containing solution and serum obtained from dogs pretreated with reserpine were weaker than those to the solutions obtained from control dogs. It may be concluded that the Hb-containing test solution releases vasoconstricting PG's from the cerebral arterial wall but vasodilating PG's from mesenteric arteries. The test solution appears to contain vasoconstricting PG's and serotonin. Serum-induced contractions appear to be mediated mainly by serotonin.

JNS + Pediatrics - 1 year subscription bundle (Individuals Only)

USD  $505.00

JNS + Pediatrics + Spine - 1 year subscription bundle (Individuals Only)

USD  $600.00

Contributor Notes

Address reprint requests to: Noboru Toda, M.D., Ph.D., Department of Pharmacology, Shiga University of Medical Sciences, Seta, Ohtsu 520–21, Japan.
  • 1.

    Alksne JF, & Smith RW: Experimental models of spasm. Clin Neurosurg 24:216227, 1977 Alksne JF, Smith RW: Experimental models of spasm. Clin Neurosurg 24:216–227, 1977

    • Search Google Scholar
    • Export Citation
  • 2.

    Allen GS, , Gold LHA, & Chou SN, et al: Cerebral arterial spasm. Part 3: In vivo intracisternal production of spasm by serotonin and blood and its reversal by phenoxybenzamine. J Neurosurg 40:451458, 1974 Allen GS, Gold LHA, Chou SN, et al: Cerebral arterial spasm. Part 3: In vivo intracisternal production of spasm by serotonin and blood and its reversal by phenoxybenzamine. J Neurosurg 40:451–458, 1974

    • Search Google Scholar
    • Export Citation
  • 3.

    Allen GS, , Henderson LM, & Chou SN, et al: Cerebral arterial spasm. Part 2: In vitro contractile activity of serotonin in human serum and CSF on the canine basilar artery, and its blockage by methysergide and phenoxybenzamine. J Neurosurg 40:442450, 1974 Allen GS, Henderson LM, Chou SN, et al: Cerebral arterial spasm. Part 2: In vitro contractile activity of serotonin in human serum and CSF on the canine basilar artery, and its blockage by methysergide and phenoxybenzamine. J Neurosurg 40:442–450, 1974

    • Search Google Scholar
    • Export Citation
  • 4.

    Arutiunov AI, , Baron MA, & Majorova NA: Experimental and clinical study of the development of spasm of the cerebral arteries related to subarachnoid hemorrhage. J Neurosurg 32:617625, 1970 Arutiunov AI, Baron MA, Majorova NA: Experimental and clinical study of the development of spasm of the cerebral arteries related to subarachnoid hemorrhage. J Neurosurg 32:617–625, 1970

    • Search Google Scholar
    • Export Citation
  • 5.

    Beitch BR, & Eakins KE: Effects of prostaglandins on the intraocular pressure of the rabbit. Br J Pharmacol 37:158167, 1969 Beitch BR, Eakins KE: Effects of prostaglandins on the intraocular pressure of the rabbit. Br J Pharmacol 37:158–167, 1969

    • Search Google Scholar
    • Export Citation
  • 6.

    Bevan JA: Response of blood vessels to sympathetic nerve stimulation. Blood Vessels 15:1725, 1978 Bevan JA: Response of blood vessels to sympathetic nerve stimulation. Blood Vessels 15:17–25, 1978

    • Search Google Scholar
    • Export Citation
  • 7.

    Bunting S, , Gryglewski R, & Moncada S, et al: Arterial walls generate from prostaglandin endoperoxides a substance (prostaglandin X) which relaxes strips of mesenteric and coeliac arteries and inhibits platelet aggregation. Prostaglandins 12:897913, 1976 Bunting S, Gryglewski R, Moncada S, et al: Arterial walls generate from prostaglandin endoperoxides a substance (prostaglandin X) which relaxes strips of mesenteric and coeliac arteries and inhibits platelet aggregation. Prostaglandins 12:897–913, 1976

    • Search Google Scholar
    • Export Citation
  • 8.

    Chokyu M: [An experimental study of cerebral vasospasm, especially on spasmogenic factors in red blood cells.] J Osaka City Med Ctr 24:211221, 1975 (Jpn) Chokyu M: [An experimental study of cerebral vasospasm, especially on spasmogenic factors in red blood cells.] J Osaka City Med Ctr 24:211–221, 1975 (Jpn)

    • Search Google Scholar
    • Export Citation
  • 9.

    Ferreira SH, & Vane Jr: New aspect of the mode of action of nonsteroid anti-inflammatory drugs. Annu Rev Pharmacol 14:5773, 1974 Ferreira SH, Vane JR: New aspect of the mode of action of nonsteroid anti-inflammatory drugs. Annu Rev Pharmacol 14:57–73, 1974

    • Search Google Scholar
    • Export Citation
  • 10.

    Fields WS, , Lemak NA, & Frankowski RF, et al: Controlled trial of aspirin in cerebral ischemia. Stroke 8:301314, 1977 Fields WS, Lemak NA, Frankowski RF, et al: Controlled trial of aspirin in cerebral ischemia. Stroke 8:301–314, 1977

    • Search Google Scholar
    • Export Citation
  • 11.

    Flamm ES, , Yasargil MG, & Ransohoff J: Control of cerebral vasospasm by parenteral phenoxybenzamine. Stroke 3:421426, 1972 Flamm ES, Yasargil MG, Ransohoff J: Control of cerebral vasospasm by parenteral phenoxybenzamine. Stroke 3:421–426, 1972

    • Search Google Scholar
    • Export Citation
  • 12.

    Flower RJ: Drugs which inhibit prostaglandin biosynthesis. Pharmacol Rev 26:3367, 1974 Flower RJ: Drugs which inhibit prostaglandin biosynthesis. Pharmacol Rev 26:33–67, 1974

    • Search Google Scholar
    • Export Citation
  • 13.

    Fraser RAR, , Stein BM, & Barrett RE, et al: Noradrenergic mediation of experimental cerebrovascular spasm. Stroke 1:356362, 1970 Fraser RAR, Stein BM, Barrett RE, et al: Noradrenergic mediation of experimental cerebrovascular spasm. Stroke 1:356–362, 1970

    • Search Google Scholar
    • Export Citation
  • 14.

    Hagen AA, , White RP, & Robertson JT: Synthesis of prostaglandins and thromboxane B2 by cerebral arteries. Stroke 10:306309, 1979 Hagen AA, White RP, Robertson JT: Synthesis of prostaglandins and thromboxane B2 by cerebral arteries. Stroke 10:306–309, 1979

    • Search Google Scholar
    • Export Citation
  • 15.

    Hayashi S, , Miyazaki M, & Toda N: Responsiveness to vasoactive agents of cerebral and mesenteric arteries isolated from control and reserpine-treated dogs. Br J Pharmacol 68:473478, 1980 Hayashi S, Miyazaki M, Toda N: Responsiveness to vasoactive agents of cerebral and mesenteric arteries isolated from control and reserpine-treated dogs. Br J Pharmacol 68:473–478, 1980

    • Search Google Scholar
    • Export Citation
  • 16.

    Kapp J, , Mahaley MS Jr, & Odom GL: Cerebral arterial spasm. Part 2: Experimental evaluation of mechanical and humoral factors in pathogenesis. J Neurosurg 29:339349, 1968 Kapp J, Mahaley MS Jr, Odom GL: Cerebral arterial spasm. Part 2: Experimental evaluation of mechanical and humoral factors in pathogenesis. J Neurosurg 29:339–349, 1968

    • Search Google Scholar
    • Export Citation
  • 17.

    Matsubara T, , Okuzono H, & Tamagawa S: Proposal for an improved reagent in the haemoglobincyanide method, in Izak G, & Lewis SM (eds): Modern Concepts of Hematology. New York/London: Academic Press, 1972, pp 2943 Matsubara T, Okuzono H, Tamagawa S: Proposal for an improved reagent in the haemoglobincyanide method, in Izak G, Lewis SM (eds): Modern Concepts of Hematology. New York/London: Academic Press, 1972, pp 29–43

    • Search Google Scholar
    • Export Citation
  • 18.

    Miyamoto T, , Ogino N, & Yamamoto S, et al: Purification of prostaglandin endoperoxide synthetase from bovine vesicular gland microsomes. J Biol Chem 251:26292636, 1976 Miyamoto T, Ogino N, Yamamoto S, et al: Purification of prostaglandin endoperoxide synthetase from bovine vesicular gland microsomes. J Biol Chem 251:2629–2636, 1976

    • Search Google Scholar
    • Export Citation
  • 19.

    Moncada S, , Gryglewski R, & Bunting S, et al: An enzyme isolated from arteries transforms prostaglandin endoperoxides to an unstable substance that inhibits platelet aggregation. Nature 263:663665, 1976 Moncada S, Gryglewski R, Bunting S, et al: An enzyme isolated from arteries transforms prostaglandin endoperoxides to an unstable substance that inhibits platelet aggregation. Nature 263:663–665, 1976

    • Search Google Scholar
    • Export Citation
  • 20.

    Pennink M, , White RP, & Crockarell JR, et al: Role of prostaglandin F in the genesis of experimental cerebral vasospasm. Angiographic study in dogs. J Neurosurg 37:398406, 1972 Pennink M, White RP, Crockarell JR, et al: Role of prostaglandin F in the genesis of experimental cerebral vasospasm. Angiographic study in dogs. J Neurosurg 37:398–406, 1972

    • Search Google Scholar
    • Export Citation
  • 21.

    Rand MJ, & Jurevics H: The pharmacology of Rauwolfia alkaloids, in Gross F (ed): Anti-hypertensive Agents. Berlin/Heidelberg/New York: Springer-Verlag, 1977, pp 77159 Rand MJ, Jurevics H: The pharmacology of Rauwolfia alkaloids, in Gross F (ed): Anti-hypertensive Agents. Berlin/Heidelberg/New York: Springer-Verlag, 1977, pp 77–159

    • Search Google Scholar
    • Export Citation
  • 22.

    Simeone FA, & Vinall P: Mechanisms of contractile response of cerebral artery to externally-applied fresh blood. J Neurosurg 43:3747, 1975 Simeone FA, Vinall P: Mechanisms of contractile response of cerebral artery to externally-applied fresh blood. J Neurosurg 43:37–47, 1975

    • Search Google Scholar
    • Export Citation
  • 23.

    Toda N: Responses of isolated cerebral and peripheral arteries to vasoconstricting agents, in Owman C, & Edvinsson L (eds): Neurogenic Control of Brain Circulation. Oxford/New York: Pergamon Press, 1977, pp 207217 Toda N: Responses of isolated cerebral and peripheral arteries to vasoconstricting agents, in Owman C, Edvinsson L (eds): Neurogenic Control of Brain Circulation. Oxford/New York: Pergamon Press, 1977, pp 207–217

    • Search Google Scholar
    • Export Citation
  • 24.

    Toda N: Responses to prostaglandins H2 and I2 of isolated dog cerebral and peripheral arteries. Am J Physiol 238:H111H117, 1980 Toda N: Responses to prostaglandins H2 and I2 of isolated dog cerebral and peripheral arteries. Am J Physiol 238:H111–H117, 1980

    • Search Google Scholar
    • Export Citation
  • 25.

    Toda N, & Fujita Y: Responsiveness of isolated cerebral and peripheral arteries to serotonin, norepinephrine, and transmural electrical stimulation. Circ Res 33:98104, 1973 Toda N, Fujita Y: Responsiveness of isolated cerebral and peripheral arteries to serotonin, norepinephrine, and transmural electrical stimulation. Circ Res 33:98–104, 1973

    • Search Google Scholar
    • Export Citation
  • 26.

    Toda N, , Hatano Y, & Hayashi S: Modification by stretches of the mechanical response of isolated cerebral and extracerebral arteries to vasoactive agents. Pfluegers Arch Eur J Physiol 374:7377, 1978 Toda N, Hatano Y, Hayashi S: Modification by stretches of the mechanical response of isolated cerebral and extracerebral arteries to vasoactive agents. Pfluegers Arch Eur J Physiol 374:73–77, 1978

    • Search Google Scholar
    • Export Citation
  • 27.

    Toda N, & Miyazaki M: Regional and species differences in the response of isolated arteries to angiotensin II. Jpn J Pharmacol 28:495497, 1978 Toda N, Miyazaki M: Regional and species differences in the response of isolated arteries to angiotensin II. Jpn J Pharmacol 28:495–497, 1978

    • Search Google Scholar
    • Export Citation
  • 28.

    Toda N, & Miyazaki M: Responses of isolated dog cerebral and peripheral arteries to prostaglandins after application of aspirin and polyphloretin phosphate. Stroke 9:490498, 1978 Toda N, Miyazaki M: Responses of isolated dog cerebral and peripheral arteries to prostaglandins after application of aspirin and polyphloretin phosphate. Stroke 9:490–498, 1978

    • Search Google Scholar
    • Export Citation
  • 29.

    Toda N, , Ozaki T, & Ohta T: Cerebrovascular sensitivity to vasoconstricting agents induced by subarachnoid hemorrhage and vasospasm in dogs. J Neurosurg 46:296303, 1977 Toda N, Ozaki T, Ohta T: Cerebrovascular sensitivity to vasoconstricting agents induced by subarachnoid hemorrhage and vasospasm in dogs. J Neurosurg 46:296–303, 1977

    • Search Google Scholar
    • Export Citation
  • 30.

    Van Assendelft OW: Spectrophotometry of Haemoglobin Derivatives. Assen, Neth: Royal Vangorcum Ltd, 1970 Van Assendelft OW: Spectrophotometry of Haemoglobin Derivatives. Assen, Neth: Royal Vangorcum Ltd, 1970

    • Search Google Scholar
    • Export Citation
  • 31.

    White RP, , Hagen AA, & Morgan H, et al: Experimental study on the genesis of cerebral vasospasm. Stroke 6:5257, 1975 White RP, Hagen AA, Morgan H, et al: Experimental study on the genesis of cerebral vasospasm. Stroke 6:52–57, 1975

    • Search Google Scholar
    • Export Citation
  • 32.

    Wilkins RH, & Levitt P: Potassium and the pathogenesis of cerebral arterial spasm in dog and man. J Neurosurg 35:4550, 1971 Wilkins RH, Levitt P: Potassium and the pathogenesis of cerebral arterial spasm in dog and man. J Neurosurg 35:45–50, 1971

    • Search Google Scholar
    • Export Citation
  • 33.

    Yamamoto YL, , Feindel W, & Wolfe LS, et al: Experimental vasoconstriction of cerebral arteries by prostaglandins. J Neurosurg 37:385397, 1972 Yamamoto YL, Feindel W, Wolfe LS, et al: Experimental vasoconstriction of cerebral arteries by prostaglandins. J Neurosurg 37:385–397, 1972

    • Search Google Scholar
    • Export Citation

Metrics

All Time Past Year Past 30 Days
Abstract Views 252 92 20
Full Text Views 158 2 0
PDF Downloads 109 2 0
EPUB Downloads 0 0 0