A transparent artificial dura mater made of silk fibroin as an inhibitor of inflammation in craniotomized rats

Laboratory investigation

Dae Won Kim Ph.D.1, Won Sik Eum Ph.D.1, Sang Ho Jang Ph.D.1, Jinseu Park Ph.D.1, Dong-Hwa Heo M.D., Ph.D.2, Seung-Hoon Sheen M.D.2, Hae-Ran Lee M.D.3, Haeyong Kweon Ph.D.4, Seok-Woo Kang Ph.D.4, Kwang-Gill Lee Ph.D.4, Se Youn Cho B.S.5, Hyoung-Joon Jin Ph.D.5, Yong-Jun Cho M.D.2, and Soo Young Choi Ph.D.1
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  • 1 Department of Biomedical Science and Research Institute for Bioscience & Biotechnology, and
  • | 2 Departments of Neurosurgery and
  • | 3 Pediatrics, Hallym University, Chuncheon;
  • | 4 Sericultural & Agicultural Materials Division, National Academy of Agricultural Science, RDA, Suwon; and
  • | 5 Department of Polymer Science and Engineering, Inha University, Incheon, Korea
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Object

To improve the safety of dura repair in neurosurgical procedures, a new dural material derived from silk fibroin was evaluated in a rat model with a dura mater injury.

Methods

The authors prepared new, transparent, artificial dura mater material using silk fibroin from the silkworm, Bombyx mori. The cytotoxic and antiinflammatory effects of the artificial dura mater were examined in vitro and in vivo by histological examination, western blotting, and reverse transcription polymerase chain reaction analyses.

Results

The novel artificial dura mater was not cytotoxic. However, it efficiently reduced cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase expression as well as the expression of the proinflammatory cytokines IL-1β, IL-6, and tumor necrosis factor–α. Cerebrospinal fluid leakage did not occur after repair of the brain of craniotomized rats with the artificial dura mater material.

Conclusions

The new artificial dura mater described in this study appears to be safe for application in neurosurgical procedures and can efficiently inhibit inflammation without side effects or CSF leakage. Although the long-term effects of this artificial dura mater material need to be validated in larger animals, the results from this study indicate that it is suitable for application in neurosurgery.

Abbreviations used in this paper:

COX-2 = cyclooxygenase-2; iNOS = inducible nitric oxide synthase; MTT = 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheyltetrazolium bromide; PBS = phosphate-buffered saline; RT-PCR = reverse transcription polymerase chain reaction; TBST = Tris-buffered saline Tween-20; TNFα = tumor necrosis factor–α.

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Contributor Notes

* Drs. Y. J. Cho and Choi contributed equally to this work.

Address correspondence to: Yong-Jun Cho, M.D., Department of Neurosurgery, Hallym University Medical Center, Chunchon 200-702, Korea. email: nssur771@hallym.or.kr.

Please include this information when citing this paper: published online October 29, 2010; DOI: 10.3171/2010.9.JNS091764.

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