Influence of interleukin-6 on the development of peritumoral brain edema in meningiomas

Laboratory investigation

Kyung-Jae Park M.D. 1 , Shin-Hyuk Kang M.D., Ph.D. 1 , Yang-Seok Chae M.D., Ph.D. 2 , Mi-Ok Yu M.S. 1 , 3 , Tai-Hyoung Cho M.D., Ph.D. 1 , Jung-Keun Suh M.D., Ph.D. 1 , Hoon-Kap Lee M.D., Ph.D. 1 and Yong-Gu Chung M.D., Ph.D. 1
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  • 1 Departments of Neurosurgery and
  • 2 Pathology, Korea University Anam Hospital, College of Medicine; and
  • 3 School of Life Sciences and Biotechnology, Korea University, Seoul, Korea
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Object

Peritumoral brain edema (PTBE) is associated with perioperative neurological deficits in patients with meningiomas. However, the pathogenesis of meningioma-associated edema remains unclear. In the present study, the authors investigated the expression of interleukin-6 (IL-6) and its relationship with PTBE in resected meningiomas.

Methods

Thirty-six benign meningiomas obtained in 36 patients were studied retrospectively. Edema volume was assessed on MR images, and an edema index (EI) was calculated. Interleukin-6 mRNA and protein expression were examined by real-time reverse transcriptase polymerase chain reaction and immunohistochemical staining.

Results

Peritumoral brain edema was found in 16 patients (44%). Neither age, sex, histological subtype, nor tumor location were related to PTBE. The level of IL-6 mRNA was 7.72 times greater in the edema group (EI > 0.2) than in the nonedema group (EI < 0.2; p = 0.011). On immunohistochemical analysis, IL-6 protein was found localized in the cytoplasm of the tumor cells, and was detected in 12 (75%) of 16 cases of edematous meningiomas, but in only 6 (30%) of 20 nonedematous cases. There was a significant correlation between the severity of PTBE and IL-6 expression (p = 0.004).

Conclusions

The authors' results in this study indicate that IL-6 expression may contribute to the development of brain edema associated with meningiomas.

Abbreviations used in this paper: BBB = blood-brain barrier; EI = edema index; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; IL-6 = interleukin-6; PCR = polymerase chain reaction; PTBE = peritumoral brain edema.

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Contributor Notes

Address correspondence to: Shin-Hyuk Kang, M.D., Ph.D., Department of Neurosurgery, Korea University Anam Hospital, College of Medicine, Korea University, 5-ga Anam dong, Sungbukgu, Seoul, 136-701, Korea. email: hermes23@kumc.or.kr.
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