Nerve transfer for restoration of lower motor neuron–lesioned bladder and urethra function: establishment of a canine model and interim pilot study results

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  • 1 Department of Computer and Electrical Engineering, College of Engineering,
  • 2 Department of Anatomy and Cell Biology, Lewis Katz School of Medicine,
  • 4 Department of Medicine, and
  • 5 Department of Urology, Temple University, Philadelphia, Pennsylvania;
  • 3 Department of Neurosurgery, Neurosurgery Paralysis Center, Massachusetts General Hospital, Boston, Massachusetts; and
  • 6 The Shriners Hospital of Philadelphia, Pennsylvania
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OBJECTIVE

Previous patient surveys have shown that patients with spinal cord or cauda equina injuries prioritize recovery of bladder function. The authors sought to determine if nerve transfer after long-term decentralization restores bladder and sphincter function in canines.

METHODS

Twenty-four female canines were included in this study. Transection of sacral roots and hypogastric nerves (S Dec) was performed in 6 animals, and 7 animals underwent this procedure with additional transection of the L7 dorsal roots (L7d+S Dec). Twelve months later, 3 L7d+S Dec animals underwent obturator-to-pelvic nerve and sciatic-to-pudendal nerve transfers (L7d+S Dec+Reinn). Eleven animals served as controls. Squat-and-void behaviors were tracked before and after decentralization, after reinnervation, and following awake bladder-filling procedures. Bladders were cystoscopically injected with Fluoro-Gold 3 weeks before euthanasia. Immediately before euthanasia, transferred nerves were stimulated to evaluate motor function. Dorsal root ganglia were assessed for retrogradely labeled neurons.

RESULTS

Transection of only sacral roots failed to reduce squat-and-void postures; L7 dorsal root transection was necessary for significant reduction. Three L7d+S Dec animals showing loss of squat-and-void postures post-decentralization were chosen for reinnervation and recovered these postures 4–6 months after reinnervation. Each showed obturator nerve stimulation–induced bladder contractions and sciatic nerve stimulation–induced anal sphincter contractions immediately prior to euthanasia. One showed sciatic nerve stimulation–induced external urethral sphincter contractions and voluntarily voided twice following nonanesthetized bladder filling. Reinnervation was confirmed by increased labeled cells in L2 and the L4–6 dorsal root ganglia (source of obturator nerve in canines) of L7d+S Dec+Reinn animals, compared with controls.

CONCLUSIONS

New neuronal pathways created by nerve transfer can restore bladder sensation and motor function in lower motor neuron–lesioned canines even 12 months after decentralization.

ABBREVIATIONS DRG = dorsal root ganglion; EUS = external urethral sphincter; L7d+S Dec = S Dec plus bilateral transection of L7 dorsal roots; L7d+S Dec+Reinn = L7d+S Dec plus reinnervation; S Dec = sacral root decentralization with exposure and bilateral transection of spinal roots below L7 and hypogastric nerves.

Supplementary Materials

    • Supplemental Table 1 (PDF 378 KB)

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Contributor Notes

Correspondence Michael R. Ruggieri Sr.: Lewis Katz School of Medicine at Temple University, Philadelphia, PA. michael.ruggieri@temple.edu.

INCLUDE WHEN CITING Published online November 8, 2019; DOI: 10.3171/2019.8.SPINE19265.

E.T. and D.M.S. contributed equally to this work.

Disclosures Dr. Pontari: consultant for Microgen Dx.

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