A remotely controlled model of spinal cord compression injury in mice: toward real-time analysis

Laboratory investigation

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  • 1 Pathophysiology and Therapy of Sensory and Motor Deficits, Institute for Neurosciences of Montpellier, INSERM U583, Saint Eloi Hospital, Montpellier;
  • 2 Department of Neurosurgery, Gui de Chauliac Hospital, Montpellier;
  • 3 Orthopedic Department, Centre Hospitalier Universitaire de Nimes;
  • 4 Neuréva Inc.-INM, Saint Eloi Hospital, Montpellier, France; and
  • 5 Neurogenomiks Laboratory, Neuroscience Department, University of the Basque Country (UPV-EHU), Leioa, Vizcaya, Spain
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Object

To date, there has been no efficient therapeutic approach to spinal cord injuries (SCIs). This may be attributable, at least in part, to difficulties in forming predictive and accurate experimental animal models. The authors' previous studies have identified 2 relevant conditions of such a model. The first condition is the ability to compare data derived from rat models of SCI by developing mouse models of SCI that permit access to a large range of transgenic models. The second condition is that the exploration of the consequences of each mechanism of spinal trauma requires modeling the different etiologic aspects of the injury.

Methods

To fulfill these 2 conditions a new model of mouse spinal cord compression injury was devised using a thread-driven olive-shaped compressive device. The authors characterized early motor, sensory, and histological outcomes using 3 olive diameters and different compression durations.

Results

A gradual and reproducible functional severity that correlated with lesion extension was demonstrated in 76 mice. To further substantiate the characterization of this model, a noncompetitive N-methyl-d-aspartate antagonist was administered in 30 mice, which demonstrated the involvement of excitotoxicity in this model.

Conclusions

The study demonstrated that spinal olive-compression injury in the mouse is a reproducible, well-characterized, and predictable model for analyzing early events after SCI. The nonmagnetic and remotely controlled design of this model will allow completion of the lesion while the animal is in the MR imaging apparatus, thus permitting further real-time MR imaging studies that will provide insights into the characterization of early events in the spatial and temporal evolution of SCI. Moreover, this model lays the foundation for future in vivo studies of functional and histological outcomes following SCI in genetically engineered animals.

Abbreviations used in this paper: NMDA = N-methyl-d-aspartate; SCI = spinal cord injury.

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Contributor Notes

Address correspondence to: Florence E. Perrin, Ph.D., Neuroscience Department, University of the Basque Country UPV/EHU, IKERBASQUE Basque Foundation for Science, E-48011 Bilbao, Spain. email:f.perrin@ikerbasque.org.
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