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Fengming Lan, Qing Qin, Huiming Yu and Xiao Yue

. 4 In this study, we sought to detect the effects of miR-448 on glycolysis and identify the relationship of glycolysis regulation to radiosensitivity in GBM. Methods Cell Culture and Transfection All human GBM cell lines and normal human astrocyte (NHA) cells used in this study were purchased from the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. Cells with DMEM supplemented with 10% fetal bovine serum (FBS) were cultured in a humidified incubator containing 5% CO 2 at 37°C. We chemically synthesized miR-448 mimics (miR-448) and miR-448