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Scott R. VandenBerg, Estelle E. May, Lucien J. Rubinstein, Mary M. Herman, E. Perentes, S. A. Vinores, V. Peter Collins, and T. S. Park

microscopy was performed using a Leitz Ortholux II epifluorescence microscope, and photographs were taken with Kodak Tri-X film at 1600 ASA. Electron Microscopic Immunocytochemistry Study A fresh surgical specimen obtained from Case 8 at the first resection was immediately placed in Waymouth's medium (as above) for transport from the operating room. Within 20 minutes of excision, the tissue was fixed according to the following protocol. It was placed for 1 hour in freshly prepared 4% paraformaldehyde with 8.5% sucrose, 0.2% glutaraldehyde, and 1 mM CaCl 2 in 0.1 M

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Daniel L. Barrow, George T. Tindall, Kalman Kovacs, Michael O. Thorner, Eva Horvath, and James C. Hoffman Jr.

-menopausal females. Pathological specimens were examined by light microscopy, immunocytochemistry, and electron microscopy. For light microscopy, the tissue was fixed in 10% buffered formalin and embedded in paraffin. Staining of sections 4 to 6 µ m was performed with hematoxylin and eosin and by the periodic acid-Schiff (PAS) method. The immunoperoxidase technique as described elsewhere 10 was used to demonstrate prolactin. For electron microscopy, tissue was fixed in 2.5% glutaraldehyde, osmicated, dehydrated in graded ethanol, processed through propylene oxide, and

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Dora W. Hsu, Fernando Hakim, Beverly M. K. Biller, Suzanne de la Monte, Nicholas T. Zervas, Anne Klibanski, and E. Tessa Hedley-Whyte

proliferating cells. Sections from five adenocarcinomas of the colon served as positive control materials for PCNA immunoreactivity. Germinal centers of the lymph nodes present in the colonic specimens provided additional positive control materials. 15 All tissues used were surgically resected, formalin-fixed, routinely processed, and paraffin-embedded. Both positive and negative control materials were included in each staining run for PCNA in pituitary tumors. Immunocytochemistry for PCNA Paraffin-embedded 6-µ sections of the initial and the recurrent biopsies were

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William J. Sonstein, Abraham Kader, W. Jost Michelsen, Josefina F. Llena, Asao Hirano, and Diana Casper

. Rabbit anti-VEGF immunoglobulin (Ig) G polyclonal antibody (Ab2; Oncogene Science, Cambridge, MA) was added to each section at a concentration of 10 |xg/ml after washing. The slides were then incubated at room temperature overnight. Negative control specimens, selected from adjacent sections of each block of tissue, were run concurrently in our immunocytochemistry protocol except that purified nonimmune rabbit IgG was replaced for the VEGF antibody or incubation with primary antibody was omitted. Specimens were processed for staining at room temperature with the

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Dora W. Hsu, Jimmy T. Efird, and E. Tessa Hedley-Whyte

characterization, specificity, and immunohistochemical staining properties of the anti-PR rat monoclonal antibody clone KD68 have been reported by Greene and Press. 17, 55 They observed a good correlation between the specific nuclear staining for PRs in tissue sections as detected by immunocytochemistry using the KD68 monoclonal antibody and the biochemically determined PR levels in tumor cytosols. The anti-ER mouse monoclonal antibody ER1D5 was raised against a 67-kD recombinant ER protein and was characterized by Al Saati, et al. 2 Hendricks and Wilkinson 23 reported a

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Rona S. Carroll, Jianping Zhang, Kathleen Dashner, Madhabananda Sar, Elizabeth M. Wilson, and Peter McL. Black

localization of estrogen receptor in target tissues using monoclonal antibodies , in Bullock GR , Petrusz P (ed): Techniques in Immunocytochemistry. New York : Academic Press , 1985 , Vol 3 , pp 43 – 54 Sar M: Application of avidin-biotin complex technique for the localization of estrogen receptor in target tissues using monoclonal antibodies, in Bullock GR, Petrusz P (ed): Techniques in Immunocytochemistry. New York: Academic Press, 1985, Vol 3, pp 43–54 54. Sar M , Lubahn DB , French FS , et al

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Jason A. Brodkey, Eric D. Laywell, Thomas F. O'Brien, Andreas Faissner, Kari Stefansson, H. Ulrich Dörries, Melitta Schachner, and Dennis A. Steindler

processed for in situ hybridization were further processed for double labeling using GFAP or neurofilament immunocytochemistry. Results Tissue samples from penetrating injuries were divided into wound, periwound, and presumed nonencumbered areas ( Fig. 1B ). Although we examined sections from all of these areas, including the wound site that often contained hemorrhagic tissue, we concentrated on periwound regions ( Figs. 2 and 3 ) because they usually represented a composite of the most and least compromised tissue states. It was our impression that a gradient

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Masahiko Tosaka, Masaru Tamura, Noboru Oriuchi, Mieko Horikoshi, Takashi Joshita, Kenichi Sugawara, Satoshi Kobayashi, Hideaki Kohga, Takatomo Yoshida, and Tomio Sasaki

. Nasiell K , Tani E , Skoog L : Fine needle aspiration cytology and immunocytochemistry of metastatic melanoma. Cytopathology 2 : 137 – 147 , 1991 Nasiell K, Tani E, Skoog L: Fine needle aspiration cytology and immunocytochemistry of metastatic melanoma. Cytopathology 2: 137–147, 1991 16. Perrot JL , Soler C , Tiffet O , et al : Detection of malignant melanoma metastasis with 99m Tc-sestamibi SPECT (single photon emission tomography) during follow-up of malignant melanoma in 17 patients. Eur J

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Pablo Bouillot, Jean-François Pellissier, Benedicte Devictor, Noel Graziani, Nicole Bianco, F. Grisoli, and Dominique Figarella-Branger

, 13 they usually found a high level of progesterone receptors (PR's) and a low level or absence of estrogen receptors (ER's). The development of immunocytochemical methods that direct monoclonal antibodies against ER's and PR's is an interesting alternative to biochemical techniques. Immunocytochemistry allows the detection of these steroid receptors at the cellular level, the comparison with histopathological features, and the assessment of intratumoral heterogeneity in terms of receptor distribution and immunostaining intensity. Furthermore, in breast cancer

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Joaquim L. Reis, Jorge Correia-Pinto, Mariana P. Monteiro, Madalena Costa, and Grover M. Hutchins

in xylene, rehydrated with grading ethanols, and stained with H & E or immunocytochemical methods. For immunocytochemistry labeling, antigen retrieval was performed by boiling the mounted section in a pressure cooker containing a 10 mM citrate buffer (pH 6.0) for 3 minutes. Samples were incubated at room temperature in 3% hydrogen peroxide in methanol to quench endogenous peroxidase, followed by normal serum of swine (DakoCytomation) for 20 minutes to block nonspecific staining. For labeling of apoptotic cells, samples were incubated with the cleaved caspase-3