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Susan A. Stern, Brian J. Zink, Michelle Mertz, Xu Wang and Steven C. Dronen

artery areas were cleaned and prepared with povidone-iodine solution. The right femoral artery was isolated via a cutdown and cannulated with a 1.67-mm-inner-diameter polyethylene catheter for blood withdrawal. The left femoral artery was similarly isolated and cannulated with a No. 5 French pigtail catheter, which was advanced into the left ventricle for BP monitoring and dye-labeled microsphere injection. The right femoral vein was cannulated with a polyethylene catheter (1.67 mm inner diameter) for drug and fluid administration. A No. 5 French flow-directed Swan

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Brian J. Zink, Susan A. Stern, Brian D. McBeth, Xu Wang and Michelle Mertz

concentration was reduced to 0.75%. The abdomen, anterior surface of the neck, and both femoral artery areas were cleaned and prepared with povidone iodine solution. The right femoral artery was isolated via a cutdown and cannulated using a 1.67-mm inner diameter polyethylene catheter for blood withdrawal. The left femoral artery was similarly isolated and cannulated using a No. 5 French pigtail catheter, which was advanced into the left ventricle for BP monitoring and dye-labeled microsphere injection. The right femoral vein was cannulated using a polyethylene catheter (1

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Brian J. Zink, Michael A. Sheinberg, Xu Wang, Michelle Mertz, Susan A. Stern and A. Lorris Betz

%) through nosecones, and endotracheally intubated. Thereafter animals were maintained on 1.15% isoflurane at a fraction of inspired oxygen rate of 28 to 31%. An orogastric tube was inserted for EtOH or placebo administration. Vascular access was obtained via cutdown of the femoral arteries and veins and the right carotid artery for blood sampling, hemorrhage, and pulmonary artery and systemic arterial pressure monitoring. A catheter was inserted into the left ventricle of the heart via the femoral artery for injection of dye-labeled microspheres to determine regional (r