✓ Microvascular end-to-end anastomoses of rat common carotid arteries measuring 0.6 to 0.7 mm in diameter were performed using an argon surgical laser system. Vascular bonding with the argon laser was accomplished in all cases. The anastomosed carotid artery segments were evaluated both angiographically and histologically at 1 day, 1 week, and 1 month after laser bonding. With increasing time after anastomosis, there was a trend toward increasing angiographically proven stenosis of the anastomotic segment and histologically demonstrated pseudoaneurysm formation of the vessel wall at the bonded site. Pseudoaneurysm formation was associated with a dense inflammatory response in the anastomotic vessel segment. In spite of excellent initial tissue bonding and vessel patency, the delayed results of progressive vessel wall disruption and segmental stenosis indicate that further experience in using the argon laser for vessel welding is needed before this method can be accepted as an alternative to current microvascular suture technique.
Stefan Pribil and Stephen K. Powers
Stephen K. Powers, Stefan Pribil, G. Yancey Gillespie III and Pamela J. Watkins
✓ The photochemotherapeutic effect of the mitochondria-specific dye rhodamine-123 (Rh-123) on human glioma cells in culture was studied. Cultured U-251MG glioma cells were incubated for 30 minutes in 10 µg/ml of Rh-123 and then exposed to blue-green light between 488 and 514.5 nm using a continuous-wave argon laser. Cells that were treated with Rh-123 and the argon laser at power densities less than 200 mW/sq cm demonstrated increasing tumor-cell killing with increasing time of exposure to laser light. Tumor-cell killing achieved with power densities of light less than 200 mW/sq cm was shown to be due solely to a photochemical effect and not to a direct (thermal) effect of the laser. The photochemical effect was dependent upon the intracellular concentration of Rh-123 and the length of light exposure, and not the intensity of light. The selective retention of Rh-123 by glioma cells and its exclusion from normal cells in conjunction with its photoactivated cytotoxicity suggest that Rh-123 may be a useful photosensitizing drug for the treatment of malignant gliomas in situ.