Plasminogen activator activity and molecular weight patterns in human brain tumors

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✓ Fresh human brain-tumor samples were assayed for their plasminogen activator (PA) content. Specific molecular weight patterns were identified for each of five common brain tumors and for normal brain, suggesting a cell-specific origin of the various PA forms. Malignant tumors contained higher PA activity and a larger number of molecular weight patterns than benign tumors, with the exception of acoustic neurinomas. Irradiated tumors contained lower PA activity than nonirradiated tumors. Finally, a slight but definite correlation between brain edema and PA activity was detected. The future role of brain-tumor PA's for diagnostic and therapeutic purposes is discussed.

Article Information

Address reprint requests to: Raymond Sawaya, M.D., Department of Neurosurgery, University of Cincinnati Medical Center, 231 Bethesda Avenue (M.L. 515), Cincinnati, Ohio 45267.

© AANS, except where prohibited by US copyright law.

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Figures

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    Protein content and PA activity of the various brain-tumortypes*

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    Zymographic analysis of extracts of various brain tumors and of normal brain. Molecular weights shown at left are in daltons.

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    Densitometric tracings of various tumor extracts and normal brain following zymographic analysis.

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    Molecular weight heterogeneity of plasminogen activators in human brain tumors. Numbers in parentheses represent the total number of samples per group; molecular weight is expressed in daltons.

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    Plasminogen activator activity in extracts of human brain tumors. Bars represent the mean activity for each tumor type.

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