Alpha-1-antitrypsin in human brain tumors

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  • 1 Departments of Neurosurgery and Physiology and Biophysics, University of Cincinnati College of Medicine, and the Veterans Administration Hospital, Cincinnati, Ohio
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✓ This study was undertaken to confirm the presence of alpha-1-antitrypsin (α1-AT) in human brain tumors and to attempt to elucidate its significance. Seventy-seven consecutive unselected patients with various brain tumors were entered in this study. The α1-AT and α2-macroglobulin contents of the tumor extracts were qualitatively assessed by Ouchterlony immunodiffusion techniques. Plasminogen activator (PA) activity was assayed electrophoretically on sodium dodecyl sulfate gels. The patients were divided into two groups according to the positivity of their tumors to α1-AT. Sixty-eight percent of the tumors were positive for α1-AT, and all specimens were negative for α2-macroglobulin. Clinical and biological parameters obtained in all study patients failed to show statistically significant differences between the two groups with the exception of PA activity (p = 0.001), the peritumoral edema as seen on computerized tomography, and the preoperative serum fibrinogen level. These three parameters were higher in the group with specimens positive to α1-AT.

This study supports the hypothesis that α1-AT is produced primarily by tumor cells in proportion to the regional proteolytic and inflammatory activity, and may protect the tumor cells.

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Address reprint requests to: Raymond Sawaya, M.D., 231 Bethesda Avenue (M.L. 515), Cincinnati, Ohio 45267.
  • 1.

    Ades EW, , Hinson A, & Chapuis-Cellier C, et al: Modulation of the immune response by plasma protease inhibitors. I. Alpha2-macroglobulin and alpha1-antitrypsin inhibit natural killing and antibody-dependent cell-mediated cytotoxicity. Scand J Immunol 15:109113, 1982 Ades EW, Hinson A, Chapuis-Cellier C, et al: Modulation of the immune response by plasma protease inhibitors. I. Alpha2-macroglobulin and alpha1-antitrypsin inhibit natural killing and antibody-dependent cell-mediated cytotoxicity. Scand J Immunol 15:109–113, 1982

    • Search Google Scholar
    • Export Citation
  • 2.

    Agostino D, & Cliffton EE: Anticoagulants and the development of pulmonary metastases. Anticoagulant effect on the Walker 256 carcinosarcoma in rats. Arch Surg 84:449453, 1962 Agostino D, Cliffton EE: Anticoagulants and the development of pulmonary metastases. Anticoagulant effect on the Walker 256 carcinosarcoma in rats. Arch Surg 84:449–453, 1962

    • Search Google Scholar
    • Export Citation
  • 3.

    Akatsuka A, , Yoshimura S, & Hatu J, et al: Intracellular localization of human plasma proteins revealed by peroxidase-antibody method in human tumors transplanted in nude mice. J Electron Microsc 28:9399, 1979 Akatsuka A, Yoshimura S, Hatu J, et al: Intracellular localization of human plasma proteins revealed by peroxidase-antibody method in human tumors transplanted in nude mice. J Electron Microsc 28:93–99, 1979

    • Search Google Scholar
    • Export Citation
  • 4.

    Astrup T: Tissue activators of plasminogen. Fed Proc 25:4251, 1966 Astrup T: Tissue activators of plasminogen. Fed Proc 25:42–51, 1966

    • Search Google Scholar
    • Export Citation
  • 5.

    Breit SN, , Wakefield D, & Robinson JP, et al: The role of α 1-antitrypsin deficiency in the pathogenesis of immune disorders. Clin Immunol Immunopathol 35:363380, 1985 Breit SN, Wakefield D, Robinson JP, et al: The role of α 1-antitrypsin deficiency in the pathogenesis of immune disorders. Clin Immunol Immunopathol 35:363–380, 1985

    • Search Google Scholar
    • Export Citation
  • 6.

    Carney DH, , Glenn KC, & Cunningham DD: Conditions which affect initiation of animal cell division by trypsin and thrombin. J Cell Physiol 95:1322, 1978 Carney DH, Glenn KC, Cunningham DD: Conditions which affect initiation of animal cell division by trypsin and thrombin. J Cell Physiol 95:13–22, 1978

    • Search Google Scholar
    • Export Citation
  • 7.

    Carrell RW, , Jeppsson JO, & Laurell CB, et al: Structure and variation of human α 1-antitrypsin. Nature 298:329334, 1982 Carrell RW, Jeppsson JO, Laurell CB, et al: Structure and variation of human α 1-antitrypsin. Nature 298:329–334, 1982

    • Search Google Scholar
    • Export Citation
  • 8.

    Dictor M: Alpha-1-antitrypsin in a malignant mixed mesodermal tumor of the ovary. Am J Surg Pathol 5:543550, 1981 Dictor M: Alpha-1-antitrypsin in a malignant mixed mesodermal tumor of the ovary. Am J Surg Pathol 5:543–550, 1981

    • Search Google Scholar
    • Export Citation
  • 9.

    Elias EG, , Sepulveda F, & Mink IB: Increasing the efficiency of cancer chemotherapy with heparin: “clinical study.” J Surg Oncol 5:189193, 1973 Elias EG, Sepulveda F, Mink IB: Increasing the efficiency of cancer chemotherapy with heparin: “clinical study.” J Surg Oncol 5:189–193, 1973

    • Search Google Scholar
    • Export Citation
  • 10.

    Galvez S, , Farcas A, & Monari M: The concentration of alpha-1-antitrypsin in cerebrospinal fluid and serum in a series of 40 intracranial tumors. Clin Chim Acta 91:191196, 1979 Galvez S, Farcas A, Monari M: The concentration of alpha-1-antitrypsin in cerebrospinal fluid and serum in a series of 40 intracranial tumors. Clin Chim Acta 91:191–196, 1979

    • Search Google Scholar
    • Export Citation
  • 11.

    Geboes K, , Ray MB, & Rutgeerts P, et al: Morphological identification of alpha-1-antitrypsin in the human small intestine. Histopathology 6:5560, 1982 Geboes K, Ray MB, Rutgeerts P, et al: Morphological identification of alpha-1-antitrypsin in the human small intestine. Histopathology 6:55–60, 1982

    • Search Google Scholar
    • Export Citation
  • 12.

    Gilbert JJ, , Paulseth JE, & Coates RK, et al: Cerebral edema associated with meningiomas. Neurosurgery 12:599605, 1983 Gilbert JJ, Paulseth JE, Coates RK, et al: Cerebral edema associated with meningiomas. Neurosurgery 12:599–605, 1983

    • Search Google Scholar
    • Export Citation
  • 13.

    Goldstein BD, , Witz G, & Amoruso M, et al: Protease inhibitors antagonize the activation of polymorphonuclear leukocyte oxygen consumption. Biochem Biophys Res Commun 88:854860, 1979 Goldstein BD, Witz G, Amoruso M, et al: Protease inhibitors antagonize the activation of polymorphonuclear leukocyte oxygen consumption. Biochem Biophys Res Commun 88:854–860, 1979

    • Search Google Scholar
    • Export Citation
  • 14.

    Highsmith RF, & Rosenberg RD: The inhibition of human plasmin by human antithrombin-heparin cofactor. J Biol Chem 249:43354338, 1974 Highsmith RF, Rosenberg RD: The inhibition of human plasmin by human antithrombin-heparin cofactor. J Biol Chem 249:4335–4338, 1974

    • Search Google Scholar
    • Export Citation
  • 15.

    Hoover HC Jr, & Ketcham AS: Techniques for inhibiting tumor metastases. Cancer 35:514, 1975 Hoover HC Jr, Ketcham AS: Techniques for inhibiting tumor metastases. Cancer 35:5–14, 1975

    • Search Google Scholar
    • Export Citation
  • 16.

    Hudig D, , Haverty T, & Fulcher C, et al: Inhibition of human natural cytotoxicity by macromolecular antiproteases. J Immunol 126:15691574, 1981 Hudig D, Haverty T, Fulcher C, et al: Inhibition of human natural cytotoxicity by macromolecular antiproteases. J Immunol 126:1569–1574, 1981

    • Search Google Scholar
    • Export Citation
  • 17.

    Janoff A, , Klassen A, & Troll W: Local vascular changes induced by the cocarcinogen, phorbol myristate acetate. Cancer Res 30:25682571, 1970 Janoff A, Klassen A, Troll W: Local vascular changes induced by the cocarcinogen, phorbol myristate acetate. Cancer Res 30:2568–2571, 1970

    • Search Google Scholar
    • Export Citation
  • 18.

    Jeppsson JO, , Laurell CB, & Fagerhol M: Properties of isolated human α 1-antitrypsin of Pi types M, S, and Z. Eur J Biochem 83:143153, 1978 Jeppsson JO, Laurell CB, Fagerhol M: Properties of isolated human α 1-antitrypsin of Pi types M, S, and Z. Eur J Biochem 83:143–153, 1978

    • Search Google Scholar
    • Export Citation
  • 19.

    Jones PA, & DeClerck YA: Destruction of extracellular matrices containing glycoproteins, elastin, and collagen by metastatic human tumor cells. Cancer Res 40:32223227, 1980 Jones PA, DeClerck YA: Destruction of extracellular matrices containing glycoproteins, elastin, and collagen by metastatic human tumor cells. Cancer Res 40:3222–3227, 1980

    • Search Google Scholar
    • Export Citation
  • 20.

    Klemi PJ, , Meurman L, & Grönroos M, et al: Clear cell (mesonephroid) tumors of the ovary with characteristics resembling endodermal sinus tumor. Int J Gynecol Pathol 1:95100, 1982 Klemi PJ, Meurman L, Grönroos M, et al: Clear cell (mesonephroid) tumors of the ovary with characteristics resembling endodermal sinus tumor. Int J Gynecol Pathol 1:95–100, 1982

    • Search Google Scholar
    • Export Citation
  • 21.

    Laug WE, , DeClerck YA, & Jones PA: Degradation of the subendothelial matrix by tumor cells. Cancer Res 43:18271834, 1983 Laug WE, DeClerck YA, Jones PA: Degradation of the subendothelial matrix by tumor cells. Cancer Res 43:1827–1834, 1983

    • Search Google Scholar
    • Export Citation
  • 22.

    Lechago J, & Fenoglio CM: Advances in Immunocytochemistry. Short Course #2. International Academy of Pathology, United States-Canadian Division, 1983 Lechago J, Fenoglio CM: Advances in Immunocytochemistry. Short Course #2. International Academy of Pathology, United States-Canadian Division, 1983

    • Search Google Scholar
    • Export Citation
  • 23.

    Liotta LA, , Abe S, & Robey PG, et al: Preferential digestion of basement membrane collagen by an enzyme derived from a metastatic murine tumor. Proc Natl Acad Sci USA 76:22682271, 1979 Liotta LA, Abe S, Robey PG, et al: Preferential digestion of basement membrane collagen by an enzyme derived from a metastatic murine tumor. Proc Natl Acad Sci USA 76:2268–2271, 1979

    • Search Google Scholar
    • Export Citation
  • 24.

    Miller RR, , Kuhlenschmidt MS, & Coffee CJ, et al: Comparison of the chemical, physical, and survival properties of normal and Z-variant α-1-antitrypsins. J Biol Chem 251:47514757, 1976 Miller RR, Kuhlenschmidt MS, Coffee CJ, et al: Comparison of the chemical, physical, and survival properties of normal and Z-variant α-1-antitrypsins. J Biol Chem 251:4751–4757, 1976

    • Search Google Scholar
    • Export Citation
  • 25.

    Mootse G, , Agostino D, & Cliffton EE: Alterations in fibrinogen, plasminogen, and inhibitors of plasmin in the growth of V2 carcinoma in rabbits. JNCI 35:567572, 1965 Mootse G, Agostino D, Cliffton EE: Alterations in fibrinogen, plasminogen, and inhibitors of plasmin in the growth of V2 carcinoma in rabbits. JNCI 35:567–572, 1965

    • Search Google Scholar
    • Export Citation
  • 26.

    Mullins DE, & Rohrlich ST: Role of proteinases in cellular invasiveness. Biochim Biophys Acta 695:177214, 1983 Mullins DE, Rohrlich ST: Role of proteinases in cellular invasiveness. Biochim Biophys Acta 695:177–214, 1983

    • Search Google Scholar
    • Export Citation
  • 27.

    Nicolson GL: Cancer metastasis. Sci Am 240:6676, 1979 Nicolson GL: Cancer metastasis. Sci Am 240:66–76, 1979

  • 28.

    Ossowski L, , Beigel D, & Reich E: Mammary plasminogen activator: correlation with involution, hormonal modulation and comparison between normal and neoplastic tissue. Cell 16:929940, 1979 Ossowski L, Beigel D, Reich E: Mammary plasminogen activator: correlation with involution, hormonal modulation and comparison between normal and neoplastic tissue. Cell 16:929–940, 1979

    • Search Google Scholar
    • Export Citation
  • 29.

    Ossowski L, , Quigley JP, & Kellerman GM, et al: Fibrinolysis associated with oncogenic transformation. Requirement of plasminogen for correlated changes in cellular morphology, colony formation in agar, and cell migration. J Exp Med 138:10561064, 1973 Ossowski L, Quigley JP, Kellerman GM, et al: Fibrinolysis associated with oncogenic transformation. Requirement of plasminogen for correlated changes in cellular morphology, colony formation in agar, and cell migration. J Exp Med 138:1056–1064, 1973

    • Search Google Scholar
    • Export Citation
  • 30.

    Ossowski L, , Unkeless JC, & Tobia A, et al: An enzymatic function associated with transformation of fibroblasts by oncogenic viruses. II. Mammalian fibroblast cultures transformed by DNA and RNA tumor viruses. J Exp Med 137:112126, 1973 Ossowski L, Unkeless JC, Tobia A, et al: An enzymatic function associated with transformation of fibroblasts by oncogenic viruses. II. Mammalian fibroblast cultures transformed by DNA and RNA tumor viruses. J Exp Med 137:112–126, 1973

    • Search Google Scholar
    • Export Citation
  • 31.

    Ouchterlony O: Diffusion-in-gel methods for immunological analysis. Prog Allergy 5:178, 1959 Ouchterlony O: Diffusion-in-gel methods for immunological analysis. Prog Allergy 5:1–78, 1959

    • Search Google Scholar
    • Export Citation
  • 32.

    Pietras RJ, , Szego CM, & Roberts JA, et al: Lysosomal cathepsin B-like activity: mobilization in prereplicative and neoplastic epithelial cells. J Histochem Cytochem 29:440450, 1981 Pietras RJ, Szego CM, Roberts JA, et al: Lysosomal cathepsin B-like activity: mobilization in prereplicative and neoplastic epithelial cells. J Histochem Cytochem 29:440–450, 1981

    • Search Google Scholar
    • Export Citation
  • 33.

    Poole AR, , Tiltman KJ, & Recklies AD, et al: Differences in secretion of the proteinase cathepsin B at the edges of human breast carcinomas and fibroadenomas. Nature 273:544547, 1978 Poole AR, Tiltman KJ, Recklies AD, et al: Differences in secretion of the proteinase cathepsin B at the edges of human breast carcinomas and fibroadenomas. Nature 273:544–547, 1978

    • Search Google Scholar
    • Export Citation
  • 34.

    Quigley JP: Association of a protease (plasminogen activator) with a specific membrane fraction isolated from transformed cells. J Cell Biol 71:472486, 1976 Quigley JP: Association of a protease (plasminogen activator) with a specific membrane fraction isolated from transformed cells. J Cell Biol 71:472–486, 1976

    • Search Google Scholar
    • Export Citation
  • 35.

    Ray MB, & Desmet VJ: Immunohistochemical demonstration of alpha-1-antitrypsin in the islet cells of human pancreas. Cell Tissue Res 187:6977, 1978 Ray MB, Desmet VJ: Immunohistochemical demonstration of alpha-1-antitrypsin in the islet cells of human pancreas. Cell Tissue Res 187:69–77, 1978

    • Search Google Scholar
    • Export Citation
  • 36.

    Ray MB, , Geboes K, & Callea F, et al: Alpha-1-antitrypsin immunoreactivity in gastric carcinoid. Histopathology 6:289297, 1982 Ray MB, Geboes K, Callea F, et al: Alpha-1-antitrypsin immunoreactivity in gastric carcinoid. Histopathology 6:289–297, 1982

    • Search Google Scholar
    • Export Citation
  • 37.

    Redelman D, & Hudig D: The mechanism of cell-mediated cytotoxicity. I. Killing by murine cytotoxic T lymphocytes requires cell surface thiols and activated proteases. J Immunol 124:870878, 1980 Redelman D, Hudig D: The mechanism of cell-mediated cytotoxicity. I. Killing by murine cytotoxic T lymphocytes requires cell surface thiols and activated proteases. J Immunol 124:870–878, 1980

    • Search Google Scholar
    • Export Citation
  • 38.

    Reich E: Activation of plasminogen: a widespread mechanism for generating localized extracellular proteolysis, in Ruddon R (ed): Biological Markers of Neoplasia: Basic and Applied Aspects. New York: Elsevier, 1978, pp 491498, Reich E: Activation of plasminogen: a widespread mechanism for generating localized extracellular proteolysis, in Ruddon R (ed): Biological Markers of Neoplasia: Basic and Applied Aspects. New York: Elsevier, 1978, pp 491–498

    • Search Google Scholar
    • Export Citation
  • 39.

    Reich E: Plasminogen activator: secretion by neoplastic cells and macrophages, in Reich E, , Rifkin DB, & Shaw E (eds): Proteases and Biological Control. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1975, pp 333341 Reich E: Plasminogen activator: secretion by neoplastic cells and macrophages, in Reich E, Rifkin DB, Shaw E (eds): Proteases and Biological Control. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1975, pp 333–341

    • Search Google Scholar
    • Export Citation
  • 40.

    Reintoft I, & Hägerstrand I: Demonstration of α 1-antitrypsin in hepatomas. Arch Pathol Lab Med 103:495498, 1979 Reintoft I, Hägerstrand I: Demonstration of α 1-antitrypsin in hepatomas. Arch Pathol Lab Med 103:495–498, 1979

    • Search Google Scholar
    • Export Citation
  • 41.

    Rifkin DB, & Crowe RM: Isolation of a protease inhibitor from tissues resistant to tumor invasion. Hoppe Seylers Z Physiol Chem 358:15251531, 1977 Rifkin DB, Crowe RM: Isolation of a protease inhibitor from tissues resistant to tumor invasion. Hoppe Seylers Z Physiol Chem 358:1525–1531, 1977

    • Search Google Scholar
    • Export Citation
  • 42.

    Ryan JJ, , Ketcham AS, & Wexler H: Reduced incidence of spontaneous metastases with long term Coumadin therapy. Ann Surg 168:163168, 1968 Ryan JJ, Ketcham AS, Wexler H: Reduced incidence of spontaneous metastases with long term Coumadin therapy. Ann Surg 168:163–168, 1968

    • Search Google Scholar
    • Export Citation
  • 43.

    Sawaya R, , Cummins CJ, & Kornblith PL: Brain tumors and plasmin inhibitors. Neurosurgery 15:795800, 1984 Sawaya R, Cummins CJ, Kornblith PL: Brain tumors and plasmin inhibitors. Neurosurgery 15:795–800, 1984

    • Search Google Scholar
    • Export Citation
  • 44.

    Sawaya R, , Mandybur T, & Ormsby I, et al: Antifibrinolytic therapy of experimentally grown malignant brain tumors. J Neurosurg 64:263268, 1986 Sawaya R, Mandybur T, Ormsby I, et al: Antifibrinolytic therapy of experimentally grown malignant brain tumors. J Neurosurg 64:263–268, 1986

    • Search Google Scholar
    • Export Citation
  • 45.

    Sheela S, & Barrett JC: In vitro degradation of radiolabelled, intact basement membrane mediated by cellular plasminogen activator. Carcinogenesis 3:363369, 1982 Sheela S, Barrett JC: In vitro degradation of radiolabelled, intact basement membrane mediated by cellular plasminogen activator. Carcinogenesis 3:363–369, 1982

    • Search Google Scholar
    • Export Citation
  • 46.

    Sloane BF, , Dunn JR, & Honn KV: Lysosomal cathepsin B: correlation with metastatic potential. Science 212:11511153, 1981 Sloane BF, Dunn JR, Honn KV: Lysosomal cathepsin B: correlation with metastatic potential. Science 212:1151–1153, 1981

    • Search Google Scholar
    • Export Citation
  • 47.

    Thornes RD: Adjuvant therapy of cancer via the cellular immune mechanism or fibrin by induced fibrinolysis and oral anticoagulants. Cancer 35:9197, 1975 Thornes RD: Adjuvant therapy of cancer via the cellular immune mechanism or fibrin by induced fibrinolysis and oral anticoagulants. Cancer 35:91–97, 1975

    • Search Google Scholar
    • Export Citation
  • 48.

    Travis J, & Salvesen GS: Human plasma proteinase inhibitors. Annu Rev Biochem 52:655709, 1983 Travis J, Salvesen GS: Human plasma proteinase inhibitors. Annu Rev Biochem 52:655–709, 1983

    • Search Google Scholar
    • Export Citation
  • 49.

    Troll W, , Frenkel K, & Wiesner R: Protease inhibitors as anticarcinogens. JNCI 73:12451250, 1984 Troll W, Frenkel K, Wiesner R: Protease inhibitors as anticarcinogens. JNCI 73:1245–1250, 1984

    • Search Google Scholar
    • Export Citation
  • 50.

    Tucker WS, , Kirsch WM, & Martinez-Hernandez A, et al: In vitro plasminogen activator activity in human brain tumors. Cancer Res 38:297302, 1978 Tucker WS, Kirsch WM, Martinez-Hernandez A, et al: In vitro plasminogen activator activity in human brain tumors. Cancer Res 38:297–302, 1978

    • Search Google Scholar
    • Export Citation
  • 51.

    Tullis JL, , Honegger H, & Fleischaker O, et al: Thrombin inhibition by malignant and normal cells: a cell-bound antithrombin effect. Cancer 48:11171182, 1981 Tullis JL, Honegger H, Fleischaker O, et al: Thrombin inhibition by malignant and normal cells: a cell-bound antithrombin effect. Cancer 48:1117–1182, 1981

    • Search Google Scholar
    • Export Citation
  • 52.

    Tuttle WC, & Jones RK: Fluorescent antibody studies of alpha-1-antitrypsin in adult human lung. Am J Clin Pathol 64:477482, 1975 Tuttle WC, Jones RK: Fluorescent antibody studies of alpha-1-antitrypsin in adult human lung. Am J Clin Pathol 64:477–482, 1975

    • Search Google Scholar
    • Export Citation
  • 53.

    Unkeless J, , Danø K, & Kellerman GM, et al: Fibrinolysis associated with oncogenic transformation. Partial purification and characterization of the cell factor, a plasminogen activator. J Biol Chem 249:42954305, 1974 Unkeless J, Danø K, Kellerman GM, et al: Fibrinolysis associated with oncogenic transformation. Partial purification and characterization of the cell factor, a plasminogen activator. J Biol Chem 249:4295–4305, 1974

    • Search Google Scholar
    • Export Citation
  • 54.

    Unkeless J, , Gordon S, & Reich E: Secretion of plasminogen activator by stimulated macrophages. J Exp Med 139:834850, 1974 Unkeless J, Gordon S, Reich E: Secretion of plasminogen activator by stimulated macrophages. J Exp Med 139:834–850, 1974

    • Search Google Scholar
    • Export Citation
  • 55.

    Unkeless J, , Tobia A, & Ossowski L, et al: An enzymatic function associated with transformation of fibroblasts by oncogenic viruses. I. Chick embryo fibroblast cultures transformed by avian RNA tumor viruses. J Exp Med 137:85112, 1973 Unkeless J, Tobia A, Ossowski L, et al: An enzymatic function associated with transformation of fibroblasts by oncogenic viruses. I. Chick embryo fibroblast cultures transformed by avian RNA tumor viruses. J Exp Med 137:85–112, 1973

    • Search Google Scholar
    • Export Citation
  • 56.

    Yoshimura S, , Tamaoki N, & Ueyama Y, et al: Plasma protein production by human tumor xenotransplanted in nude mice. Cancer Res 38:34743478, 1978 Yoshimura S, Tamaoki N, Ueyama Y, et al: Plasma protein production by human tumor xenotransplanted in nude mice. Cancer Res 38:3474–3478, 1978

    • Search Google Scholar
    • Export Citation

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