Complement fixation by IgM and IgG autoantibodies on cultured human glial cells

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✓ The humoral immune response to autologous gliomas has been investigated in 16 patients using immune adherence and anti-C3 immunofluorescence assays of serum samples. Significant antiglioma antibody activity was detected in 56% of these sera. Fractionation experiments demonstrated that IgM class antibodies were more effective than IgG in their ability to fix complement on cultured glioma cell membranes. In addition, autologous IgG was shown to inhibit IgM complement-fixing activity in some experiments. “Capping” of immune complexes on the glioma cell surface is also described.

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Contributor Notes

Present address for Dr. Garson: Frenchay Hospital, Department of Neurology, Bristol, England.Address reprint requests to: Eugene A. Quindlen, M.D., Building 10 A, Room 3E68, National Institutes of Health, Bethesda, Maryland 20205.

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