Effect of slosh mitigation on histologic markers of traumatic brain injury
Laboratory investigation
Search for other papers by Ryan C. Turner in
Current site
Google Scholar
PubMed
Search for other papers by Zachary J. Naser in
Current site
Google Scholar
PubMed
Search for other papers by Julian E. Bailes in
Current site
Google Scholar
PubMed
Search for other papers by David W. Smith in
Current site
Google Scholar
PubMed
Search for other papers by Joseph A. Fisher in
Current site
Google Scholar
PubMed
Search for other papers by Charles L. Rosen in
Current site
Google Scholar
PubMed
Object
Helmets successfully prevent most cranial fractures and skull traumas, but traumatic brain injury (TBI) and concussions continue to occur with frightening frequency despite the widespread use of helmets on the athletic field and battlefield. Protection against such injury is needed. The object of this study was to determine if slosh mitigation reduces neural degeneration, gliosis, and neuroinflammation.
Methods
Two groups of 10 adult male Sprague-Dawley rats were subjected to impact-acceleration TBI. One group of animals was fitted with a collar inducing internal jugular vein (IJV) compression prior to injury, whereas the second group received no such collar prior to injury. All rats were killed 7 days postinjury, and the brains were fixed and embedded in paraffin. Tissue sections were processed and stained for markers of neural degeneration (Fluoro-Jade B), gliosis (glial fibrillary acidic protein), and neuroinflammation (ionized calcium binding adapter molecule 1).
Results
Compared with the controls, animals that had undergone IJV compression had a 48.7%–59.1% reduction in degenerative neurons, a 36.8%–45.7% decrease in reactive astrocytes, and a 44.1%–65.3% reduction in microglial activation.
Conclusions
The authors concluded that IJV compression, a form of slosh mitigation, markedly reduces markers of neurological injury in a common model of TBI. Based on findings in this and other studies, slosh mitigation may have potential for preventing TBI in the clinical population.
Abbreviations used in this paper:
DPBS = Dulbecco phosphate-buffered saline; FJB = Fluoro-Jade B; GFAP = glial fibrillary acidic protein; Iba-1 = ionized calcium binding adapter molecule 1; ICP = intracranial pressure; IJV = internal jugular vein; TBI = traumatic brain injury.
© Copyright 1944-2023 American Association of Neurological Surgeons