A novel tissue engineering approach using an endothelial progenitor cell–seeded biopolymer to treat intracranial saccular aneurysms

Laboratory investigation

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Object

Recurrence after endovascular coiling of intracranial aneurysms is reported in up to 42% of cases and is attributed to the lack of endothelialization across the neck. In this study the authors used a novel tissue engineering approach to promote endothelialization by seeding endothelial progenitor cells (EPCs) within a fibrin polymer injected endovascularly into the aneurysm.

Methods

Experimental aneurysms were created in New Zealand White rabbits and were left untreated, surgically clipped, or embolized with platinum coils, fibrin biopolymer alone, or fibrin combined with autologous cultured EPCs.

Results

In aneurysms treated with EPCs, a confluent monolayer of endothelial cells with underlying neointima was demonstrated across the neck at 16 weeks posttreatment, which was not observed with aneurysms treated using the other methods.

Conclusions

This novel technique may address reasons for the limited durability of standard coil embolization and provides further avenues for the development of improved devices for the care of patients with aneurysms.

Abbreviations used in this paper:DiI-Ac-LDL = acetylated low-density lipoprotein labeled with 1,1′-dioctadecyl-3,3,3′,3′,-tetramethylindocarbocyanine perchlorate; EGM = endothelial growth medium; EPC = endothelial progenitor cell; GDC = Guglielmi detachable coil; PECAM-1 = platelet/endothelial cell adhesion molecule 1.
Article Information

Contributor Notes

Address correspondence to: Christopher S. Ogilvy, M.D., Department of Neurosurgery, Massachusetts General Hospital, 15 Parkman Street, Wang Building, Room 745, Boston, Massachusetts 02114. email: cogilvy@partners.org.* Drs. Aronson and Mitha contributed equally to this work.Please include this information when citing this paper: published online June 29, 2012; DOI: 10.3171/2012.5.JNS091308.

© AANS, except where prohibited by US copyright law.

Headings
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