Accuracy of reinnervation is an important factor that determines outcome after nerve injury and repair. Type grouping—the clustering of muscle fibers of the same type after reinnervation—can be used to investigate the accuracy of reinnervation. In this study, the degree of type grouping after crush injury in rats was compared with the clustering of muscle fibers after autografting or single-lumen nerve grafting.
Twelve weeks after sciatic nerve crush injury in rats, clustering of Type I muscle fibers was analyzed in the target muscle with adenosine 5′-triphosphatase staining. In addition, the number of regenerated axons was determined in the nerve distal to the crush injury. Results were compared with that of the authors' previous study.
Type grouping was more abundant after crush injury than after autograft or single-lumen nerve graft repair.
Crush injury leads to more clustered innervation of muscle fibers, probably because the Schwann cell basal lamina tubes are not interrupted as they are in autograft or artificial nerve graft repair. This finding adds to understanding the processes playing a role in nerve regeneration.
Abbreviations used in this paper: CSA = cross-sectional area; TMC = trimethylene carbonate.
Drs. Bovenberg and Degeling contributed equally to this study.
Address correspondence to: Carmen L. A. M. Vleggeert-Lankamp, Ph.D., M.D., Department of Neurosurgery, Leiden University Medical Centre, P.O. Box 9600, RC Leiden, The Netherlands NL-2300. email: email@example.com.
Please include this information when citing this paper: published online November 26, 2010; DOI: 10.3171/2010.9.JNS091656.
Accioli-De-VaconcellosZAKassar-DuchossoyLMiraJC: Long term evaluation of experimental median nerve repair by frozen and fresh nerve autografts, allografts and allografts repopulated by autologous Schwann cells. Restor Neurol Neurosci15:17–241999
BertelliJATalebMMiraJCCalixtoJB: Muscle fiber type reorganization and behavioral functional recovery of rat median nerve repair with vascularized or conventional nerve grafts. Restor Neurol Neurosci10:5–121996
FeirabendHKChoufoerHVoogdJ: White matter of the cerebellum of the chicken (Gallus domesticus): a quantitative light and electron microscopic analysis of myelinated fibers and fiber compartments. J Comp Neurol369:236–2511996
FeirabendHKKokPChoufoerHPloegerS: Preservation of myelinated fibers for electron microscopy: a qualitative comparison of aldehyde fixation, microwave stabilisation and other procedures all completed by osmication. J Neurosci Methods55:137–1531994
FeirabendHKP: Anatomy and Development of Longitudinal Patterns in the Architecture of the Cerebellum of the White Leghorn (Gallus Domesticus) [doctoral thesis]Leiden, The NetherlandsUniversity of Leiden1983
GillespieMJGordonTMurphyPR: Motor units and histochemistry in rat lateral gastrocnemius and soleus muscles: evidence for dissociation of physiological and histochemical properties after reinnervation. J Neurophysiol57:921–9371987
KugelbergEEdströmLAbbruzzeseM: Mapping of motor units in experimentally reinnervated rat muscle. Interpretation of histochemical and atrophic fibre patterns in neurogenic lesions. J Neurol Neurosurg Psychiatry33:319–3291970
MagillCKTongAKawamuraDHayashiAHunterDAParsadanianA: Reinnervation of the tibialis anterior following sciatic nerve crush injury: a confocal microscopic study in transgenic mice. Exp Neurol207:64–742007
VarejãoASCabritaAMMeekMFBulas-CruzJMelo-PintoPRaimondoS: Functional and morphological assessment of a standardized rat sciatic nerve crush injury with a non-serrated clamp. J Neurotrauma21:1652–16702004
Vleggeert-LankampCLvan den BergRJFeirabendHKLakkeEAMalessyMJThomeerRT: Electrophysiology and morphometry of the Aalpha- and Abeta-fiber populations in the normal and regenerating rat sciatic nerve. Exp Neurol187:337–3492004