Comonitoring of adenosine and dopamine using the Wireless Instantaneous Neurotransmitter Concentration System: proof of principle

Laboratory investigation

Young-Min Shon M.D., Ph.D.1,4, Su-Youne Chang Ph.D.1, Susannah J. Tye Ph.D.1, Christopher J. Kimble M.A.2, Kevin E. Bennet B.S.Ch.E., M.B.A.2, Charles D. Blaha Ph.D.5, and Kendall H. Lee M.D., Ph.D.1,3
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  • 1 Department of Neurologic Surgery,
  • | 2 Division of Engineering, and
  • | 3 Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota;
  • | 4 Department of Neurology, The Catholic University of Korea, Seoul, Korea; and
  • | 5 Department of Psychology, University of Memphis, Tennessee
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Object

The authors of previous studies have demonstrated that local adenosine efflux may contribute to the therapeutic mechanism of action of thalamic deep brain stimulation (DBS) for essential tremor. Real-time monitoring of the neurochemical output of DBS-targeted regions may thus advance functional neurosurgical procedures by identifying candidate neurotransmitters and neuromodulators involved in the physiological effects of DBS. This would in turn permit the development of a method of chemically guided placement of DBS electrodes in vivo. Designed in compliance with FDA-recognized standards for medical electrical device safety, the authors report on the utility of the Wireless Instantaneous Neurotransmitter Concentration System (WINCS) for real-time comonitoring of electrical stimulation–evoked adenosine and dopamine efflux in vivo, utilizing fast-scan cyclic voltammetry (FSCV) at a polyacrylonitrile-based (T-650) carbon fiber microelectrode (CFM).

Methods

The WINCS was used for FSCV, which consisted of a triangle wave scanned between −0.4 and +1.5 V at a rate of 400 V/second and applied at 10 Hz. All voltages applied to the CFM were with respect to an Ag/AgCl reference electrode. The CFM was constructed by aspirating a single T-650 carbon fiber (r = 2.5 μm) into a glass capillary and pulling to a microscopic tip using a pipette puller. The exposed carbon fiber (the sensing region) extended beyond the glass insulation by ~ 50 μm. Proof of principle tests included in vitro measurements of adenosine and dopamine, as well as in vivo measurements in urethane-anesthetized rats by monitoring adenosine and dopamine efflux in the dorsomedial caudate putamen evoked by high-frequency electrical stimulation of the ventral tegmental area and substantia nigra.

Results

The WINCS provided reliable, high-fidelity measurements of adenosine efflux. Peak oxidative currents appeared at +1.5 V and at +1.0 V for adenosine, separate from the peak oxidative current at +0.6 V for dopamine. The WINCS detected subsecond adenosine and dopamine efflux in the caudate putamen at an implanted CFM during high-frequency stimulation of the ventral tegmental area and substantia nigra. Both in vitro and in vivo testing demonstrated that WINCS can detect adenosine in the presence of other easily oxidizable neurochemicals such as dopamine comparable to the detection abilities of a conventional hardwired electrochemical system for FSCV.

Conclusions

Altogether, these results demonstrate that WINCS is well suited for wireless monitoring of high-frequency stimulation-evoked changes in brain extracellular concentrations of adenosine. Clinical applications of selective adenosine measurements may prove important to the future development of DBS technology.

Abbreviations used in this paper:

AP = anteroposterior; ATP = adenosine triphosphate; CFM = carbon fiber microelectrode; CPu = caudate putamen; DBS = deep brain stimulation; DV = dorsoventral; FSCV = fast-scan cyclic voltammetry; ML = mediolateral; VTA/SN = ventral tegmental area and substantia nigra; WINCS = Wireless Instantaneous Neurotransmitter Concentration System.

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