Immunohistochemical expression of nestin in adenohypophysial vessels during development of pituitary infarction

Laboratory investigation

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  • 1 Departments of Laboratory Medicine and
  • 2 Neurosurgery, St. Michael's Hospital, University of Toronto, Ontario, Canada; and
  • 3 Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota
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Object

The aim of this work was to investigate the immunohistochemical expression of nestin, a member of the intermediate filament family, in adenohypophysial vasculature during development and progression of pituitary infarction.

Methods

Forty-five nontumorous adenohypophyses and 34 pituitary adenomas of various types, all exhibiting acute or healing infarcts, were examined immunohistochemically using the streptavidin-biotin-peroxidase complex method.

Results

In both adenohypophyses and pituitary adenomas without infarction, nestin was expressed in only a few capillaries and endothelial cells. In acute infarcts without a vascular response, no nestin was demonstrable within necrotic capillaries (50 cases). In organizing infarcts, newly formed vessels spreading into necrotic zones showed nestin expression in all capillaries and practically every endothelial cell (25 cases). In the hypocellular, fibrotic scar phase, only a few vessels (4) were apparent, and immunoreactivity was focal and mild.

Conclusions

Nestin is strongly expressed in newly formed capillaries and is downregulated when infarcts transform to fibrous tissue. Nestin expression may provide valuable insight into the process of pituitary angiogenesis.

Abbreviations used in this paper:CNS = central nervous system; GFP = green fluorescent protein; RFP = red fluorescent protein; VEGF = vascular endothelial growth factor.

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Contributor Notes

Address correspondence to: Bernd W. Scheithauer, M.D., Mayo Clinic, Department of Laboratory Medicine and Pathology, 200 First Street SW, Rochester, Minnesota 55905. email: scheithauer.bernd@mayo.edu.
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